Evidence map›Paper›PMID 36716395›Full record

ArticleACS synthetic biology2023

Silencing Transcription from an Influenza Reverse Genetics Plasmid in

Tahir Malik, Laura Klenow, Alexandros Karyolaimos, Jan-Willem de Gier, Robert Daniels

Open access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in ACS synthetic biology, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
1.1field-weighted citation impact, top 23% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 5 citations in OpenAlex.

  1. Journal of virology · 2025
    Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 2 institutions in 2 countries.

Tahir MalikDivision of Viral Products, Center for Biologics Evaluation and Research, Food and Drug Administration, Silver Spring, Maryland 20993, United States.
Laura KlenowDivision of Viral Products, Center for Biologics Evaluation and Research, Food and Drug Administration, Silver Spring, Maryland 20993, United States.
Alexandros KaryolaimosDepartment of Biochemistry and Biophysics, Stockholm University, 10691 Stockholm, Sweden.
Jan-Willem de GierDepartment of Biochemistry and Biophysics, Stockholm University, 10691 Stockholm, Sweden.
Robert DanielsDivision of Viral Products, Center for Biologics Evaluation and Research, Food and Drug Administration, Silver Spring, Maryland 20993, United States.ORCID 0000-0002-5988-2920
Center for Biologics Evaluation and Research · USStockholm University · SE

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Reverse genetics (RG) systems have been instrumental for determining the molecular aspects of viral replication, pathogenesis, and for the development of therapeutics. Here, we demonstrate that genes encoding the influenza surface antigens hemagglutinin and neuraminidase have varying stability when cloned into a common RG plasmid and transformed into

Indexed as

Escherichia coliInfluenza, HumanHumansLac OperonLac RepressorsPlasmidsReverse GeneticsLac Repressorscloning toxic genesHAhemagglutininIAVsinfluenza A virusinfluenza virus rescueiVEClac operonNAneuraminidaseregulatory elements

Identifiers

PMID36716395
PMCPMC9942234
OpenAlexW4318579505

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.