ArticleMethods in molecular biology (Clifton, N.J.)2023
Optimized Fixation and Phalloidin Staining of Basally Localized F-Actin Networks in Collectively Migrating Follicle Cells.
Article in Methods in molecular biology (Clifton, N.J.), 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
3 citing papers in PubMed, 5 citations in OpenAlex.
- A complex relationship between the architecture of the basement membrane, its mechanical properties, and its ability to shape the Drosophila egg.Matrix biology : journal of the International Society for Matrix Biology · 2025Article
- Research of Optical Properties and Biocompatibility in Different Zones of Multilayered Translucent Zirconia on Hydrothermal Aging.Materials (Basel, Switzerland) · 2024Article
- Fat2 polarizes Lar and Sema5c to coordinate the motility of collectively migrating epithelial cells.Journal of cell science · 2024Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
The follicular epithelial cells of the Drosophila egg chamber have become a premier model to study how cells globally orient their actin-based machinery for collective migration. The basal surface of each follicle cell has lamellipodial and filopodial protrusions that extend from its leading edge and an array of stress fibers that mediate its adhesion to the extracellular matrix; these migratory structures are all globally aligned in the direction of tissue movement. To understand how this global alignment is achieved, one must be able to reliably visualize the underlying F-actin; however, dynamic F-actin networks can be difficult to preserve in fixed tissues. Here, we describe an optimized protocol for the fixation and phalloidin staining of the follicular epithelium. We also provide a brief primer on relevant aspects of the image acquisition process to ensure high quality data are collected.
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Registered trials
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