ArticlePlant physiology2023
U-box E3 ubiquitin ligase PUB8 attenuates abscisic acid responses during early seedling growth.
Article in Plant physiology, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 30 citations in OpenAlex.
- The EAR motif-containing adaptor protein ECAP enhances ABI5-mediated ABA signaling to inhibit seed germination in Arabidopsis.Plant communications · 2026Article
- E3 ligase ATL17 is a key regulator of abscisic acid signaling that mediates AHG1 degradation in Arabidopsis thaliana.The New phytologist · 2026Article
- Ubiquitination-Mediated Regulation of Brassinosteroid Signaling.Plants (Basel, Switzerland) · 2026Review
- SnRK2.6-mediated phosphorylation stabilizes proteasome regulator 1 (PTRE1) to enhance 26S proteasome activity and coordinate ABA signaling in Arabidopsis.Plant communications · 2026Article
- Holistic overview of regulatory networks governing seed dormancy and germination in plants.Science China. Life sciences · 2026Review
- Identification and characterization of U-box E3 ubiquitin ligase genes (ChPUBs) in Cardamine hupingshanensis under abiotic stress.BMC genomics · 2026Article
- A 14-3-3 modulator of seed weight and quality for unlocking the yield potential of soybean.Nature communications · 2025Article
- Cell death-inducing DNA fragmentation factor alpha (DFFA)-like effectors (CIDEs) improve lipid droplet (LD) formation and oil accumulation in plant tissues.Plant biotechnology journal · 2025Article
- The RAE1-STOP1 module regulates ABA sensitivity in early seedlings of Arabidopsis.BMC plant biology · 2025Article
- Map-based cloning of Zmccr3 and its network construction and validation for regulating maize seed germination.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2025Article
- Review
- Nitrate attenuates abscisic acid signaling via NIN-LIKE PROTEIN8 in Arabidopsis seed germination.The Plant cell · 2025Article
- The U-box E3 ubiquitin ligase PUB35 negatively regulates ABA signaling through AFP1-mediated degradation of ABI5.The Plant cell · 2024Article
- INDUCER OF CBF EXPRESSION 1 promotes cold-enhanced immunity by directly activating salicylic acid signaling.The Plant cell · 2024Article
- Research Progress in the Regulation of the ABA Signaling Pathway by E3 Ubiquitin Ligases in Plants.International journal of molecular sciences · 2024Review
- Apple E3 ligase MdPUB23 mediates ubiquitin-dependent degradation of MdABI5 to delay ABA-triggered leaf senescence.Horticulture research · 2024Article
- Genome-Wide Mining of CULLIN E3 Ubiquitin Ligase Genes fromPlants (Basel, Switzerland) · 2024Article
Corrections and comments
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Authors and funding
8 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
ABSCISIC ACID-INSENSITIVE3 (ABI3) and ABI5 are 2 crucial transcription factors in abscisic acid (ABA) signaling, and their homeostasis at the protein level plays a decisive role in seed germination and subsequent seedling growth. Here, we found that PLANT U-BOX 8 (PUB8), a U-box E3 ubiquitin ligase, physically interacts with ABI3 and ABI5 and negatively regulates ABA responses during early Arabidopsis (Arabidopsis thaliana) seedling growth. Loss-of-function pub8 mutants were hypersensitive to ABA-inhibited cotyledon greening, while lines overexpressing PUB8 with low levels of ABI5 protein abundance were insensitive to ABA. Genetic analyses showed that ABI3 and ABI5 were required for the ABA-sensitive phenotype of pub8, indicating that PUB8 functions upstream of ABI3 and ABI5 to regulate ABA responses. Biochemical analyses showed that PUB8 can associate with ABI3 and ABI5 for degradation through the ubiquitin-mediated 26S proteasome pathway. Correspondingly, loss-of-function of PUB8 led to enhanced ABI3 and ABI5 stability, while overexpression of PUB8 impaired accumulation of ABI3 and ABI5 in planta. Further phenotypic analysis indicated that PUB8 compromised the function of ABI5 during early seedling growth. Taken together, our results reveal the regulatory role of PUB8 in modulating the early seedling growth by controlling the homeostasis of ABI3 and ABI5.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.