Evidence map›Paper›PMID 36715020›Full record

ArticleDevelopment (Cambridge, England)2023

Single-cell RNA-based phenotyping reveals a pivotal role of thyroid hormone receptor alpha for hypothalamic development.

Varun K A Sreenivasan, Riccardo Dore, Julia Resch, Julia Maier, Carola Dietrich, Jana Henck, Saranya Balachandran, Jens Mittag, Malte Spielmann

Open access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Development (Cambridge, England), 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.

0numbers the graph read from it
0cells of the map it votes in
7citing papers in PubMed
2.6field-weighted citation impact, top 9% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

7 citing papers in PubMed, 13 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 2 institutions in 1 country.

Varun K A SreenivasanInstitute of Human Genetics, Universitätsklinikum Schleswig-Holstein, University of Lübeck and University of Kiel, Lübeck 23562, Germany.ORCID 0000-0002-8667-8394
Riccardo DoreInstitute for Endocrinology and Diabetes, University of Lübeck and Universitätsklinikum Schleswig-Holstein Campus Lübeck, Center of Brain Behavior and Metabolism (CBBM), Ratzeburger Allee 160, 23562 Lübeck, Germany.
Julia ReschInstitute for Endocrinology and Diabetes, University of Lübeck and Universitätsklinikum Schleswig-Holstein Campus Lübeck, Center of Brain Behavior and Metabolism (CBBM), Ratzeburger Allee 160, 23562 Lübeck, Germany.
Julia MaierInstitute for Endocrinology and Diabetes, University of Lübeck and Universitätsklinikum Schleswig-Holstein Campus Lübeck, Center of Brain Behavior and Metabolism (CBBM), Ratzeburger Allee 160, 23562 Lübeck, Germany.
Carola DietrichHuman Molecular Genomics Group, Max Planck Institute for Molecular Genetics, Berlin 14195, Germany.
Jana HenckInstitute of Human Genetics, Universitätsklinikum Schleswig-Holstein, University of Lübeck and University of Kiel, Lübeck 23562, Germany.
Saranya BalachandranInstitute of Human Genetics, Universitätsklinikum Schleswig-Holstein, University of Lübeck and University of Kiel, Lübeck 23562, Germany.
Jens MittagInstitute for Endocrinology and Diabetes, University of Lübeck and Universitätsklinikum Schleswig-Holstein Campus Lübeck, Center of Brain Behavior and Metabolism (CBBM), Ratzeburger Allee 160, 23562 Lübeck, Germany.ORCID 0000-0001-7778-5158
Malte SpielmannInstitute of Human Genetics, Universitätsklinikum Schleswig-Holstein, University of Lübeck and University of Kiel, Lübeck 23562, Germany.ORCID 0000-0002-0583-4683
University Hospital Schleswig-Holstein · DEMax Planck Institute for Molecular Genetics · DE

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Thyroid hormone and its receptor TRα1 play an important role in brain development. Several animal models have been used to investigate this function, including mice heterozygous for the TRα1R384C mutation, which confers receptor-mediated hypothyroidism. These mice display abnormalities in several autonomic functions, which was partially attributed to a developmental defect in hypothalamic parvalbumin neurons. However, whether other cell types in the hypothalamus are similarly affected remains unknown. Here, we used single-nucleus RNA sequencing to obtain an unbiased view on the importance of TRα1 for hypothalamic development and cellular diversity. Our data show that defective TRα1 signaling has surprisingly little effect on the development of hypothalamic neuronal populations, but it heavily affects hypothalamic oligodendrocytes. Using selective reactivation of the mutant TRα1 during specific developmental periods, we find that early postnatal thyroid hormone action seems to be crucial for proper hypothalamic oligodendrocyte maturation. Taken together, our findings underline the well-known importance of postnatal thyroid health for brain development and provide an unbiased roadmap for the identification of cellular targets of TRα1 action in mouse hypothalamic development.

Indexed as

RNAThyroid Hormone Receptors alphaAnimalsHypothalamusMiceThyroid GlandThyroid HormonesRNAThyroid Hormone Receptors alphaThyroid HormonesHypothalamusHypothyroidismMouseOligodendrocyteSingle-cell sequencingThyroid receptor

Identifiers

PMID36715020
PMCPMC10110490
OpenAlexW4318472760

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.