Evidence map›Paper›PMID 36714845›Full record

ArticlePNAS nexus2022

Mosquito tagging using DNA-barcoded nanoporous protein microcrystals.

Julius D Stuart, Daniel A Hartman, Lyndsey I Gray, Alec A Jones, Natalie R Wickenkamp, Christine Hirt, Aya Safira, April R Regas, Therese M Kondash, Margaret L Yates and 3 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in PNAS nexus, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.

0numbers the graph read from it
0cells of the map it votes in
10citing papers in PubMed
2.0field-weighted citation impact, top 15% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

10 citing papers in PubMed, 11 citations in OpenAlex.

  1. Article
  2. Article
  3. Article
  4. Article
  5. Article
  6. Article
  7. Review
  8. Article
  9. Review
  10. Scalable Combinatorial Assembly of Synthetic DNA for Tracking Applications.International journal of molecular sciences · 2023
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors at 2 institutions in 1 country.

Julius D StuartDepartment of Chemistry, Colorado State University, Fort Collins, CO 80523, USA.ORCID https://orcid.org/0000-0003-0214-4481
Daniel A HartmanDepartment of Microbiology, Immunology, and Pathology, Colorado State University, Fort Collins, CO 80523, USA.ORCID https://orcid.org/0000-0001-9733-8355
Lyndsey I GrayDepartment of Microbiology, Immunology, and Pathology, Colorado State University, Fort Collins, CO 80523, USA.ORCID https://orcid.org/0000-0001-5897-464X
Alec A JonesSchool of Biomedical Engineering, Colorado State University, Fort Collins, CO 80523, USA.ORCID https://orcid.org/0000-0003-1667-9882
Natalie R WickenkampDepartment of Microbiology, Immunology, and Pathology, Colorado State University, Fort Collins, CO 80523, USA.ORCID https://orcid.org/0000-0001-6748-2903
Christine HirtDepartment of Microbiology, Immunology, and Pathology, Colorado State University, Fort Collins, CO 80523, USA.
Aya SafiraSchool of Biomedical Engineering, Colorado State University, Fort Collins, CO 80523, USA.
April R RegasCollege of Veterinary Medicine and Biological Sciences, Colorado State University, Fort Collins, CO 80523, USA.ORCID https://orcid.org/0000-0002-4534-3432
Therese M KondashDepartment of Environmental Health and Radiological Sciences, Colorado State University, Fort Collins, CO 80523, USA.ORCID https://orcid.org/0000-0002-9475-6532
Margaret L YatesDepartment of Biochemistry and Molecular Biology, Colorado State University, Fort Collins, CO 80523, USA.ORCID https://orcid.org/0000-0003-3018-8457
Sergei DrigaDepartment of Chemical and Biological Engineering, Colorado State University, Fort Collins, Colorado 80523, USA.
Christopher D SnowDepartment of Chemistry, Colorado State University, Fort Collins, CO 80523, USA.ORCID https://orcid.org/0000-0002-7690-3519
Rebekah C KadingORCID https://orcid.org/0000-0002-4996-915X
Colorado State University · USCornell University · US

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Conventional mosquito marking technology for mark-release-recapture (MRR) is quite limited in terms of information capacity and efficacy. To overcome both challenges, we have engineered, lab-tested, and field-evaluated a new class of marker particles, in which synthetic, short DNA oligonucleotides (DNA barcodes) are adsorbed and protected within tough, crosslinked porous protein microcrystals. Mosquitoes self-mark through ingestion of microcrystals in their larval habitat. Barcoded microcrystals persist trans-stadially through mosquito development if ingested by larvae, do not significantly affect adult mosquito survivorship, and individual barcoded mosquitoes are detectable in pools of up to at least 20 mosquitoes. We have also demonstrated crystal persistence following adult mosquito ingestion. Barcode sequences can be recovered by qPCR and next-generation sequencing (NGS) without detectable amplification of native mosquito DNA. These DNA-laden protein microcrystals have the potential to radically increase the amount of information obtained from future MRR studies compared to previous studies employing conventional mosquito marking materials.

Indexed as

host-guest crystalsmark–release–recapturemosquito surveillanceporous protein crystalsvector biology

Identifiers

PMID36714845
PMCPMC9802479
OpenAlexW4295632926

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.