Evidence map›Paper›PMID 36712940›Full record

ArticlePNAS nexus2023

Senescent stroma induces nuclear deformations in cancer cells via the inhibition of RhoA/ROCK/myosin II-based cytoskeletal tension.

Ivie Aifuwa, Byoung Choul Kim, Pratik Kamat, Bartholomew Starich, Anshika Agrawal, Derin Tanrioven, Teresa R Luperchio, Angela M Jimenez Valencia, Tania Perestrelo, Karen Reddy and 2 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in PNAS nexus, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.

0numbers the graph read from it
0cells of the map it votes in
8citing papers in PubMed
1.4field-weighted citation impact, top 18% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

8 citing papers in PubMed, 12 citations in OpenAlex.

  1. Article
  2. Article
  3. Review
  4. Indentation induces instantaneous nuclear stiffening and unfolding of nuclear envelope wrinkles.Proceedings of the National Academy of Sciences of the United States of America · 2023
    Article
  5. Article
  6. [Advances in cell nuclear mechanobiology and its regulation mechanisms].Sheng wu yi xue gong cheng xue za zhi = Journal of biomedical engineering = Shengwu yixue gongchengxue zazhi · 2023
    Review
  7. Review
  8. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors at 2 institutions in 2 countries.

Ivie AifuwaJohns Hopkins Physical Sciences - Oncology Center, Institute for Nanobiotechnology, Johns Hopkins University, Baltimore, MD 21218, USA.
Byoung Choul KimDepartment of Biomedical Engineering, Johns Hopkins University, Baltimore, MD 21218, USA.
Pratik KamatJohns Hopkins Physical Sciences - Oncology Center, Institute for Nanobiotechnology, Johns Hopkins University, Baltimore, MD 21218, USA.
Bartholomew StarichJohns Hopkins Physical Sciences - Oncology Center, Institute for Nanobiotechnology, Johns Hopkins University, Baltimore, MD 21218, USA.
Anshika AgrawalJohns Hopkins Physical Sciences - Oncology Center, Institute for Nanobiotechnology, Johns Hopkins University, Baltimore, MD 21218, USA.
Derin TanriovenJohns Hopkins Physical Sciences - Oncology Center, Institute for Nanobiotechnology, Johns Hopkins University, Baltimore, MD 21218, USA.
Teresa R LuperchioDepartment of Biological Chemistry, Johns Hopkins University, Baltimore, MD 21218, USA.
Angela M Jimenez ValenciaJohns Hopkins Physical Sciences - Oncology Center, Institute for Nanobiotechnology, Johns Hopkins University, Baltimore, MD 21218, USA.
Tania PerestreloJohns Hopkins Physical Sciences - Oncology Center, Institute for Nanobiotechnology, Johns Hopkins University, Baltimore, MD 21218, USA.
Karen ReddyDepartment of Biological Chemistry, Johns Hopkins University, Baltimore, MD 21218, USA.
Taekjip HaDepartment of Biomedical Engineering, Johns Hopkins University, Baltimore, MD 21218, USA.
Jude M PhilipJohns Hopkins Physical Sciences - Oncology Center, Institute for Nanobiotechnology, Johns Hopkins University, Baltimore, MD 21218, USA.
Johns Hopkins University · USIncheon National University · KR

Funding

Technological Assessment and Solutions Core - RC4P30AG021334 · NIA · JOHNS HOPKINS UNIVERSITY · PI Jeremy D Walston · 2003 to 2026
$33.2M
Validation of Nuclear Morphology as a Biomarker of Aging and Aging-Related PhenotypesU01AG060903 · NIA · JOHNS HOPKINS UNIVERSITY · PI WIRTZ, DENIS · 2018 to 2022
$3.1M
NIA NIH HHS P30 AG021334NIA NIH HHS U01 AG060903
6 · The paper itself

Abstract

The presence of senescent cells within tissues has been functionally linked to malignant transformations. Here, using tension-gauge tethers technology, particle-tracking microrheology, and quantitative microscopy, we demonstrate that senescent-associated secretory phenotype (SASP) derived from senescent fibroblasts impose nuclear lobulations and volume shrinkage on malignant cells, which stems from the loss of RhoA/ROCK/myosin II-based cortical tension. This loss in cytoskeletal tension induces decreased cellular contractility, adhesion, and increased mechanical compliance. These SASP-induced morphological changes are, in part, mediated by Lamin A/C. These findings suggest that SASP induces defective outside-in mechanotransduction from actomyosin fibers in the cytoplasm to the nuclear lamina, thereby triggering a cascade of biophysical and biomolecular changes in cells that associate with malignant transformations.

Identifiers

PMID36712940
PMCPMC9830950
OpenAlexW4310793328

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.