ArticleFrontiers in genetics2022
Combining methods for non-invasive fecal DNA enables whole genome and metagenomic analyses in wildlife biology.
Article in Frontiers in genetics, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
13 citing papers in PubMed, 26 citations in OpenAlex.
- Cross-Species Sex Identification and Comparative Analysis of theAnimals : an open access journal from MDPI · 2026Article
- Habitat-Associated Dietary Plasticity in the Japanese Weasel (Animals : an open access journal from MDPI · 2026Article
- Article
- DNA purity over yield: optimizing extraction and PCR in degraded Asian elephant (Elephas maximus) fecal samples.Genes & genomics · 2026Article
- Development of Metagenomic Methods for Health Monitoring of Endangered Species Using Fecal Samples.Evolutionary applications · 2026Article
- Review
- Veterinary medicine in reintroduction and reinforcement of the European pond turtle Emys orbicularis: a review.BMC veterinary research · 2025Review
- 16S rRNA and Metagenomic Datasets of Gastrointestinal Microbiota in Fetal and 7-Day-Old Goat Kids.Scientific data · 2025Article
- Avoidance of reproductive conflict and the evolution of menopause in chimpanzees.Royal Society open science · 2025Article
- Comparative Metagenomic Analysis of the Gut Microbiota of Captive Pangolins: A Case Study of Two Species.Animals : an open access journal from MDPI · 2024Article
- Similarities and differences: species and diet impact gut microbiota of captive pheasants.PeerJ · 2024Article
- An experimental design for obtaining DNA of a target species and its diet from a single non-invasive genetic protocol.Ecology and evolution · 2023Article
- Genetic diversity in global populations of the critically endangered addax (Evolutionary applications · 2023Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors at 3 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Non-invasive biological samples benefit studies that investigate rare, elusive, endangered, or dangerous species. Integrating genomic techniques that use non-invasive biological sampling with advances in computational approaches can benefit and inform wildlife conservation and management. Here, we used non-invasive fecal DNA samples to generate low- to medium-coverage genomes (e.g., >90% of the complete nuclear genome at six X-fold coverage) and metagenomic sequences, combining widely available and accessible DNA collection cards with commonly used DNA extraction and library building approaches. DNA preservation cards are easy to transport and can be stored non-refrigerated, avoiding cumbersome or costly sample methods. The genomic library construction and shotgun sequencing approach did not require enrichment or targeted DNA amplification. The utility and potential of the data generated was demonstrated through genome scale and metagenomic analyses of zoo and free-ranging African savanna elephants (
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.