Evidence map›Paper›PMID 36712070›Full record

ArticlebioRxiv : the preprint server for biology2023

CTCF is essential for proper mitotic spindle structure and anaphase segregation.

Katherine Chiu, Yasmin Berrada, Nebiyat Eskndir, Dasol Song, Claire Fong, Sarah Naughton, Tina Chen, Savanna Moy, Sarah Gyurmey, Liam James and 16 more

Open access · greenAbstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in bioRxiv : the preprint server for biology, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 4 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

26 authors at 2 institutions in 2 countries.

Katherine ChiuBiology Department, University of Massachusetts Amherst, Amherst, MA 01003, USA.
Yasmin BerradaBiology Department, University of Massachusetts Amherst, Amherst, MA 01003, USA.
Nebiyat EskndirBiology Department, University of Massachusetts Amherst, Amherst, MA 01003, USA.
Dasol SongBiology Department, University of Massachusetts Amherst, Amherst, MA 01003, USA.
Claire FongBiology Department, University of Massachusetts Amherst, Amherst, MA 01003, USA.
Sarah NaughtonBiology Department, University of Massachusetts Amherst, Amherst, MA 01003, USA.
Tina ChenBiology Department, University of Massachusetts Amherst, Amherst, MA 01003, USA.
Savanna MoyBiology Department, University of Massachusetts Amherst, Amherst, MA 01003, USA.
Sarah GyurmeyBiology Department, University of Massachusetts Amherst, Amherst, MA 01003, USA.
Liam JamesBiology Department, University of Massachusetts Amherst, Amherst, MA 01003, USA.
Chimere EzeiruakuBiology Department, University of Massachusetts Amherst, Amherst, MA 01003, USA.
Caroline CapistranBiology Department, University of Massachusetts Amherst, Amherst, MA 01003, USA.
Daniel LoweyBiology Department, University of Massachusetts Amherst, Amherst, MA 01003, USA.
Vedang DiwanjiBiology Department, University of Massachusetts Amherst, Amherst, MA 01003, USA.
Samantha PetersonBiology Department, University of Massachusetts Amherst, Amherst, MA 01003, USA.
Harshini ParakhBiology Department, University of Massachusetts Amherst, Amherst, MA 01003, USA.
Ayanna R BurgessBiology Department, University of Massachusetts Amherst, Amherst, MA 01003, USA.
Cassandra ProbertBiology Department, University of Massachusetts Amherst, Amherst, MA 01003, USA.
Annie ZhuBiology Department, University of Massachusetts Amherst, Amherst, MA 01003, USA.
Bryn AndersonBiology Department, University of Massachusetts Amherst, Amherst, MA 01003, USA.
Nehora LeviBiology Department of Molecular Biology, Faculty of Life Sciences, Ariel University, Ariel 40700, Israel.
Gabi GerlitzBiology Department of Molecular Biology, Faculty of Life Sciences, Ariel University, Ariel 40700, Israel.
Mary C PackardBiology Department, University of Massachusetts Amherst, Amherst, MA 01003, USA.
Katherine A DorfmanBiology Department, University of Massachusetts Amherst, Amherst, MA 01003, USA.
Michael Seifu BahiruBiology Department, University of Massachusetts Amherst, Amherst, MA 01003, USA.
Andrew D StephensBiology Department, University of Massachusetts Amherst, Amherst, MA 01003, USA.
University of Massachusetts Amherst · USAriel University · IL

Funding

Center for 3D Structure and Physics of the GenomeUM1HG011536 · NHGRI · UNIV OF MASSACHUSETTS MED SCH WORCESTER · PI DEKKER, JOB, MIRNY, LEONID A · 2020 to 2024
$11.8M
Analyzing the role of chromatin compaction in nuclear mechanics, structure, and functionR00GM123195 · NIGMS · UNIVERSITY OF MASSACHUSETTS AMHERST · PI STEPHENS, ANDREW DANIEL · 2020 to 2022
$745k
NHGRI NIH HHS UM1 HG011536NIGMS NIH HHS R00 GM123195
6 · The paper itself

Abstract

Mitosis is an essential process in which the duplicated genome is segregated equally into two daughter cells. CTCF has been reported to be present in mitosis but its importance for mitotic fidelity remains to be determined. To evaluate the importance of CTCF in mitosis, we tracked mitotic behaviors in wild type and two different CTCF CRISPR-based genetic knockdowns. We find that knockdown of CTCF results in prolonged mitoses and failed anaphase segregation via time lapse imaging of SiR-DNA. CTCF knockdown did not alter cell cycling or the mitotic checkpoint, which was activated upon nocodazole treatment. Immunofluorescence imaging of the mitotic spindle in CTCF knockdowns revealed disorganization via tri/tetrapolar spindles and chromosomes behind the spindle pole. Imaging of interphase nuclei showed that nuclear size increased drastically, consistent with failure to divide the duplicated genome in anaphase. Population measurements of nuclear shape in CTCF knockdowns do not display decreased circularity or increased nuclear blebbing relative to wild type. However, failed mitoses do display abnormal nuclear morphologies relative to successful mitoses, suggesting population images do not capture individual behaviors. Thus, CTCF is important for both proper metaphase organization and anaphase segregation which impacts the size and shape of the interphase nucleus.

Identifiers

PMID36712070
PMCPMC9881978
OpenAlexW4315489658

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.