Evidence map›Paper›PMID 36711806›Full record

ArticlebioRxiv : the preprint server for biology2023

Adaptive optical two-photon fluorescence microscopy probes cellular organization of ocular lenses in vivo.

Santosh Kumar Paidi, Qinrong Zhang, Yuhan Yang, Chun-Hong Xia, Na Ji, Xiaohua Gong

Open access · greenAbstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in bioRxiv : the preprint server for biology, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 0 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

6 authors at 2 institutions in 1 country.

Santosh Kumar PaidiSchool of Optometry, University of California, Berkeley, California 94720, USA.
Qinrong ZhangDepartment of Physics, University of California, Berkeley, California 94720, USA.
Yuhan YangDepartment of Physics, University of California, Berkeley, California 94720, USA.
Chun-Hong XiaSchool of Optometry, University of California, Berkeley, California 94720, USA.
Na JiDepartment of Physics, University of California, Berkeley, California 94720, USA.
Xiaohua GongSchool of Optometry, University of California, Berkeley, California 94720, USA.
University of California, Berkeley · USLawrence Berkeley National Laboratory · US

Funding

UC Berkeley Vision Science COREP30EY003176 · NEI · UNIVERSITY OF CALIFORNIA BERKELEY · PI RICHARD H KRAMER · 1985 to 2026
$16.4M
Cataracts, Connexin Mutants and Genetic ModifiersR01EY013849 · NEI · UNIVERSITY OF CALIFORNIA BERKELEY · PI Xiaohua Gong · 2002 to 2026
$8.2M
Lens epithelial cell heterogeneity during developmentR01EY031253 · NEI · UNIVERSITY OF CALIFORNIA BERKELEY · PI GONG, XIAOHUA · 2020 to 2024
$1.8M
Adaptive optical microscopy for high-accuracy recording of neural activity in vivoU01NS118300 · NINDS · UNIVERSITY OF CALIFORNIA BERKELEY · PI JI, NA · 2021 to 2023
$1.7M
NEI NIH HHS P30 EY003176NEI NIH HHS R01 EY013849NEI NIH HHS R01 EY031253NINDS NIH HHS U01 NS118300
6 · The paper itself

Abstract

The mammalian ocular lens is an avascular multicellular organ that grows continuously throughout life. Traditionally, its cellular organization is investigated using dissected lenses, which eliminates in vivo environmental and structural support. Here, we demonstrated that two-photon fluorescence microscopy (2PFM) can visualize lens cells in vivo. To maintain subcellular resolution at depth, we employed adaptive optics (AO) to correct aberrations due to ocular and lens tissues, which led to substantial signal and resolution improvements. Imaging lens cells up to 980 μm deep, we observed novel cellular organizations including suture-associated voids, enlarged vacuoles, and large cavities, contrary to the conventional view of a highly ordered organization. We tracked these features longitudinally over weeks and observed the incorporation of new cells during growth. Taken together, non-invasive longitudinal in vivo imaging of lens morphology using AO 2PFM will allow us to directly observe the development or alterations of lens cellular organization in living animals.

Identifiers

PMID36711806
PMCPMC9882239
OpenAlexW4317488981

What OpenQuestion holds

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LicenceCC BY-NC-ND
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.