Evidence map›Paper›PMID 36711804›Full record

ArticlebioRxiv : the preprint server for biology2023

Transposon mutagenesis libraries reveal novel molecular requirements during CRISPR RNA-guided DNA integration.

Matt W G Walker, Sanne E Klompe, Dennis J Zhang, Samuel H Sternberg

Open access · greenAbstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in bioRxiv : the preprint server for biology, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 12 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

4 authors at 1 institution in 1 country.

Matt W G WalkerDepartment of Biological Sciences, Columbia University, New York, NY, 10027, USA.
Sanne E KlompeDepartment of Biochemistry and Molecular Biophysics, Columbia University, New York, NY, 10032, USA.
Dennis J ZhangDepartment of Biological Sciences, Columbia University, New York, NY, 10027, USA.
Samuel H SternbergDepartment of Biochemistry and Molecular Biophysics, Columbia University, New York, NY, 10032, USA.
Columbia University · US

Funding

Leveraging Programmable Integrases for Human Genome EngineeringDP2HG011650 · NHGRI · COLUMBIA UNIVERSITY HEALTH SCIENCES · PI STERNBERG, SAMUEL HENRY · 2020 to 2020
$2.4M
Impact of CRISPR-associated transposons on anti-phage immunity in Vibrio choleraeR21AI168976 · NIAID · COLUMBIA UNIVERSITY HEALTH SCIENCES · PI STERNBERG, SAMUEL HENRY · 2022 to 2023
$446k
NHGRI NIH HHS DP2 HG011650NIAID NIH HHS R21 AI168976
6 · The paper itself

Abstract

CRISPR-associated transposons (CASTs) direct DNA integration downstream of target sites using the RNA-guided DNA binding activity of nuclease-deficient CRISPR-Cas systems. Transposition relies on several key protein-protein and protein-DNA interactions, but little is known about the explicit sequence requirements governing efficient transposon DNA integration activity. Here, we exploit pooled library screening and high-throughput sequencing to reveal novel sequence determinants during transposition by the Type I-F

Identifiers

PMID36711804
PMCPMC9882353
OpenAlexW4317493231

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.