Evidence map›Paper›PMID 36711785›Full record

ArticleResearch square2023

Spatiotemporal immune atlas of the first clinical-grade, gene-edited pig-to-human kidney xenotransplant.

Matthew D Cheung, Rebecca Asiimwe, Elise N Erman, Christopher F Fucile, Shanrun Liu, Chiao-Wang Sun, Vidya Sagar Hanumanthu, Harish C Pal, Emma D Wright, Gelare Ghajar-Rahimi and 18 more

Open access · greenAbstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in Research square, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 3 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

28 authors at 1 institution in 1 country.

Matthew D CheungDepartment of Surgery, University of Alabama at Birmingham; Birmingham, AL, USA.ORCID 0000-0003-4182-5220
Rebecca AsiimweDepartment of Surgery, University of Alabama at Birmingham; Birmingham, AL, USA.
Elise N ErmanDepartment of Surgery, University of Alabama at Birmingham; Birmingham, AL, USA.
Christopher F FucileInformatics Institute, University of Alabama at Birmingham; Birmingham, AL, USA.
Shanrun LiuDepartment of Biochemistry and Molecular Genetics, University of Alabama at Birmingham; Birmingham, AL, USA.
Chiao-Wang SunDepartment of Biochemistry and Molecular Genetics, University of Alabama at Birmingham; Birmingham, AL, USA.
Vidya Sagar HanumanthuFlow Cytometry & Single Cell Core Facility, University of Alabama at Birmingham; Birmingham, AL, USA.
Harish C PalFlow Cytometry & Single Cell Core Facility, University of Alabama at Birmingham; Birmingham, AL, USA.
Emma D WrightDepartment of Surgery, University of Alabama at Birmingham; Birmingham, AL, USA.
Gelare Ghajar-RahimiDepartment of Surgery, University of Alabama at Birmingham; Birmingham, AL, USA.ORCID 0000-0001-5089-7176
Daniel EpsteinDepartment of Surgery, University of Alabama at Birmingham; Birmingham, AL, USA.
Babak J OrandiDepartment of Surgery, University of Alabama at Birmingham; Birmingham, AL, USA.ORCID 0000-0001-6026-7135
Vineeta KumarDepartment of Medicine, University of Alabama at Birmingham; Birmingham, AL, USA.ORCID 0000-0002-4271-463X
Douglas J AndersonDepartment of Surgery, University of Alabama at Birmingham; Birmingham, AL, USA.
Morgan E GreeneDepartment of Surgery, University of Alabama at Birmingham; Birmingham, AL, USA.
Markayla BellDepartment of Surgery, University of Alabama at Birmingham; Birmingham, AL, USA.
Stefani YatesDepartment of Surgery, University of Alabama at Birmingham; Birmingham, AL, USA.
Kyle H MooreDepartment of Medicine, University of Alabama at Birmingham; Birmingham, AL, USA.
Jennifer LaFontaineDepartment of Surgery, University of Alabama at Birmingham; Birmingham, AL, USA.
John T KillianDepartment of Surgery, University of Alabama at Birmingham; Birmingham, AL, USA.ORCID 0000-0002-3702-9005
Gavin BakerDepartment of Surgery, University of Alabama at Birmingham; Birmingham, AL, USA.
Jackson PerryDepartment of Surgery, University of Alabama at Birmingham; Birmingham, AL, USA.
Rhiannon ReedDepartment of Surgery, University of Alabama at Birmingham; Birmingham, AL, USA.
Shawn C LittleDepartment of Surgery, University of Alabama at Birmingham; Birmingham, AL, USA.
Alexander F RosenbergInformatics Institute, University of Alabama at Birmingham; Birmingham, AL, USA.
James F GeorgeDepartment of Surgery, University of Alabama at Birmingham; Birmingham, AL, USA.
Jayme E LockeDepartment of Surgery, University of Alabama at Birmingham; Birmingham, AL, USA.
Paige M PorrettDepartment of Surgery, University of Alabama at Birmingham; Birmingham, AL, USA.ORCID 0000-0002-6895-5597
University of Alabama at Birmingham · US

Funding

MEDICAL SCIENTIST TRAINING PROGRAMT32GM008361 · NIGMS · UNIVERSITY OF ALABAMA AT BIRMINGHAM · PI YACOUBIAN, TALENE ALENE · 1992 to 2024
$16.8M
IMMUNOLOGIC DISEASES AND BASIC IMMUNOLOGYT32AI007051 · NIAID · UNIVERSITY OF ALABAMA AT BIRMINGHAM · PI Laurie Ellen Harrington · 1985 to 2026
$13.1M
TRAINING IN CELLULAR PHYSIOLOGY MEMBRANE TRANSPORTT32DK007545 · NIDDK · UNIVERSITY OF ALABAMA AT BIRMINGHAM · PI GUTIERREZ, ORLANDO M · 1987 to 2023
$4.5M
PRedoctoral Interdisciplinary training in renal physiology and MEdicine (PRIME)T32DK116672 · NIDDK · UNIVERSITY OF ALABAMA AT BIRMINGHAM · PI POLLOCK, JENNIFER S · 2018 to 2022
$761k
NIAID NIH HHS T32 AI007051NIDDK NIH HHS T32 DK007545NIDDK NIH HHS T32 DK116672NIGMS NIH HHS T32 GM008361
6 · The paper itself

Abstract

Pig-to-human xenotransplantation is rapidly approaching the clinical arena; however, it is unclear which immunomodulatory regimens will effectively control human immune responses to pig xenografts. We transplanted a gene-edited pig kidney into a brain-dead human recipient on pharmacologic immunosuppression and studied the human immune response to the xenograft using spatial transcriptomics and single-cell RNA sequencing. Human immune cells were uncommon in the porcine kidney cortex early after xenotransplantation and consisted of primarily myeloid cells. Both the porcine resident macrophages and human infiltrating macrophages expressed genes consistent with an alternatively activated, anti-inflammatory phenotype. No significant infiltration of human B or T cells into the porcine kidney xenograft was detected. Altogether, these findings provide proof of concept that conventional pharmacologic immunosuppression is sufficient to restrict infiltration of human immune cells into the xenograft early after compatible pig-to-human kidney xenotransplantation.

Identifiers

PMID36711785
PMCPMC9882594
OpenAlexW4313811994

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.