Evidence map›Paper›PMID 36711480›Full record

ArticlebioRxiv : the preprint server for biology2023

Secretion encoded single-cell sequencing (SEC-seq) uncovers gene expression signatures associated with high VEGF-A secretion in mesenchymal stromal cells.

Shreya Udani, Justin Langerman, Doyeon Koo, Sevana Baghdasarian, Brian Cheng, Simran Kang, Citradewi Soemardy, Joseph de Rutte, Kathrin Plath, Dino Di Carlo

Open access · greenAbstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in bioRxiv : the preprint server for biology, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 7 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

10 authors at 2 institutions in 1 country.

Shreya UdaniDepartment of Bioengineering, University of California - Los Angeles, Los Angeles, CA 90095, USA.
Justin LangermanDepartment of Biological Chemistry, David Geffen School of Medicine, University of California - Los Angeles, Los Angeles, CA 90095, USA.
Doyeon KooDepartment of Bioengineering, University of California - Los Angeles, Los Angeles, CA 90095, USA.
Sevana BaghdasarianDepartment of Chemical and Biomolecular Engineering, University of California - Los Angeles, Los Angeles, CA 90095, USA.
Brian ChengDepartment of Bioengineering, University of California - Los Angeles, Los Angeles, CA 90095, USA.
Simran KangDepartment of Bioengineering, University of California - Los Angeles, Los Angeles, CA 90095, USA.
Citradewi SoemardyDepartment of Bioengineering, University of California - Los Angeles, Los Angeles, CA 90095, USA.
Joseph de RuttePartillion Bioscience, Los Angeles, CA 90095, USA.
Kathrin PlathDepartment of Biological Chemistry, David Geffen School of Medicine, University of California - Los Angeles, Los Angeles, CA 90095, USA.
Dino Di CarloDepartment of Bioengineering, University of California - Los Angeles, Los Angeles, CA 90095, USA.
University of California, Los Angeles · USCalifornia NanoSystems Institute · US

Funding

Women's CancersP30CA016042 · NCI · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI Samuel Wheeler French · 1985 to 2026
$134.5M
Roles of sumoylation and alternative polyadenylation in cellular reprogrammingP01GM099134 · NIGMS · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI PLATH, KATHRIN · 2011 to 2021
$20.3M
Enhancing the potency of mesenchymal stem cell therapies for kidney diseases using lab-on-a-particle technologyR21DK128730 · NIDDK · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI DI CARLO, DINO · 2021 to 2021
$185k
NCI NIH HHS P30 CA016042NIDDK NIH HHS R21 DK128730NIGMS NIH HHS P01 GM099134
6 · The paper itself

Abstract

Cells secrete numerous bioactive molecules essential for the function of healthy organisms. However, there are no scalable methods to link individual cell secretions to their transcriptional state. By developing and using secretion encoded single-cell sequencing (SEC-seq), which exploits hydrogel nanovials to capture individual cells and their secretions, we simultaneously measured the secretion of vascular endothelial growth factor A (VEGF-A) and the transcriptome for thousands of individual mesenchymal stromal cells (MSCs). We found that VEGF-A secretion is heterogeneous across the cell population and lowly correlated with the

Identifiers

PMID36711480
PMCPMC9881958
OpenAlexW4313750735

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.