Evidence map›Paper›PMID 36709241›Full record

ArticleScientific reports2023

Expression of Chrna9 is regulated by Tbx3 in undifferentiated pluripotent stem cells.

Takashi Yazawa, Yoshitaka Imamichi, Takeshi Kitano, Mohammad Sayful Islam, Md Rafiqul Islam Khan, Satoru Takahashi, Toshio Sekiguchi, Nobuo Suzuki, Akihiro Umezawa, Junsuke Uwada

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Scientific reports, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
0.6field-weighted citation impact, top 31% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 4 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 7 institutions in 2 countries.

Takashi YazawaDepartment of Biochemistry, Asahikawa Medical University, Midorigaoka Higashi 2-1-1-1, Asahikawa, Hokkaido, 078-8510, Japan. yazawa@asahikawa-med.ac.jp.
Yoshitaka ImamichiDepartment of Marine Science and Technology, Faculty of Marine Science and Technology, Fukui Prefectural University, Fukui, 917-0003, Japan.
Takeshi KitanoDepartment of Biological Sciences, Graduate School of Science and Technology, Kumamoto University, Kumamoto, 860-8555, Japan.
Mohammad Sayful IslamDepartment of Biochemistry, Asahikawa Medical University, Midorigaoka Higashi 2-1-1-1, Asahikawa, Hokkaido, 078-8510, Japan.
Md Rafiqul Islam KhanDepartment of Biochemistry, Asahikawa Medical University, Midorigaoka Higashi 2-1-1-1, Asahikawa, Hokkaido, 078-8510, Japan.
Satoru TakahashiDepartment of Pediatrics, Asahikawa Medical University, Midorigaoka Higashi 2-1-1-1, Asahikawa, Hokkaido, 078-8510, Japan.
Toshio SekiguchiNoto Marine Laboratory, Division of Marine Environmental Studies, Institute of Nature and Environmental Technology, Kanazawa University, Noto-cho, Ishikawa, 927-0553, Japan.
Nobuo SuzukiNoto Marine Laboratory, Division of Marine Environmental Studies, Institute of Nature and Environmental Technology, Kanazawa University, Noto-cho, Ishikawa, 927-0553, Japan.
Akihiro UmezawaDepartment of Reproduction, National Center for Child Health and Development Research Institute, Setagaya, Tokyo, 157-8535, Japan.
Junsuke UwadaDepartment of Biochemistry, Asahikawa Medical University, Midorigaoka Higashi 2-1-1-1, Asahikawa, Hokkaido, 078-8510, Japan.
Asahikawa Medical University · JPKanazawa University · JPFukui Prefectural University · JPKanazawa Medical University · JPKumamoto University · JPNational Center For Child Health and Development · JPUniversity of Rajshahi · BD

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

It was reported that nicotinic acetylcholine receptor (nAChR)-mediated signaling pathways affect the proliferation and differentiation of pluripotent stem cells. However, detail expression profiles of nAChR genes were unrevealed in these cells. In this study, we comprehensively investigated the gene expression of α subunit of nAChRs (Chrna) during differentiation and induction of pluripotent stem cells. Mouse embryonic stem (ES) cells expressed multiple Chrna genes (Chrna3-5, 7 and 9) in undifferentiated status. Among them, Chrna9 was markedly down-regulated upon the differentiation into mesenchymal cell lineage. In mouse tissues and cells, Chrna9 was mainly expressed in testes, ES cells and embryonal F9 teratocarcinoma stem cells. Expression of Chrna9 gene was acutely reduced during differentiation of ES and F9 cells within 24 h. In contrast, Chrna9 expression was increased in induced pluripotent stem cells established from mouse embryonic fibroblast. It was shown by the reporter assays that T element-like sequence in the promoter region of Chrna9 gene is important for its activities in ES cells. Chrna9 was markedly reduced by siRNA-mediated knockdown of Tbx3, a pluripotency-related transcription factor of the T-box gene family. These results indicate that Chrna9 is a nAChR gene that are transcriptionally regulated by Tbx3 in undifferentiated pluripotent cells.

Indexed as

Pluripotent Stem CellsReceptors, NicotinicT-Box Domain ProteinsAnimalsCell DifferentiationEmbryonic Stem CellsFibroblastsMiceTranscription FactorsChrna9 protein, mouseReceptors, NicotinicT-Box Domain ProteinsTbx3 protein, mouseTranscription Factors

Identifiers

PMID36709241
PMCPMC9884305
OpenAlexW4318336629

What OpenQuestion holds

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LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.