ArticleBiotechnology letters2023
swnk plays an important role in the biosynthesis of swainsonine in Metarhizium anisopliae.
Article in Biotechnology letters, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
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Who cites it
7 citing papers in PubMed, 7 citations in OpenAlex.
- Genomic features and the transcriptional regulation of secondary metabolite biosynthesis of endophytic fungusMicrobial genomics · 2026Article
- Endophytic Alternaria oxytropis modulates host metabolism and enhances stress resilience in locoweed independent of swainsonine biosynthesis.BMC plant biology · 2026Article
- siRNA-Silencing ofFrontiers in microbiology · 2025Article
- Analysis of the Mycotoxin Levels and Expression Pattern of SWN Genes at Different Time Points in the FungusMicroorganisms · 2024Article
- Transcriptomic Screening ofJournal of fungi (Basel, Switzerland) · 2024Article
- Establishment of RNA Interference Genetic Transformation System and Functional Analysis of FlbA Gene inInternational journal of molecular sciences · 2023Article
- Construction of Yeast One-Hybrid Library ofJournal of fungi (Basel, Switzerland) · 2023Article
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Authors and funding
8 authors at 3 institutions in 1 country.
Funding
Abstract
objectiveSwainsonine (SW) is the principal toxic ingredient of locoweeds, and is produced by multiple fungi. A key enzyme in the SW synthesis pathway is a hybrid swnk/nrps. To analyze the role of swnk in the SW biosynthesis pathway of Metarhizium anisopliae.
resultsThe concentration of SW and the swnk expression in M. anisopliae fermentation from 1st to 7th day were determined using LC-MS and RT-qPCR, respectively. M. anisopliae had the highest SW content and swnk expression on the 5th day of fermentation; Mutant strain (MT) were obtained by PEG-mediated homologous recombination (HR) which knocked out swnk in the wild-type (WT) strain. Complemented-type (CT) strain were obtained by transforming a modified PUC19 complementation vector containing the geneticin (G418) resistance gene and swnK. SW was not detected in the MT strain and reverted to its original level in the CT strain; A Psilent-1 plasmid with Benomyl (ben)-resistant that was used interfered with swnk of WT strain. The level of SW was markedly diminished in the RNAi strain. RNAi of swnk affects the formation of the cell wall in M. anisopliae.
conclusionThese results indicate that swnk plays a crucial role in the SW biosynthesis of M. anisopliae.
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