ArticleNature communications2023
Highly efficient and robust π-FISH rainbow for multiplexed in situ detection of diverse biomolecules.
Article in Nature communications, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
19 citing papers in PubMed, 51 citations in OpenAlex.
- In Situ Amplified Mutational mRNA Imaging Using a Spatially Confined CRISPR Nanoplatform.Angewandte Chemie (International ed. in English) · 2026Article
- PTH-induced behavioral and metabolic alterations in mouse models of hyperparathyroidism.iScience · 2026Article
- Dissecting human organ development using spatial technologies.Developmental cell · 2026Review
- Vision of Life's Code: Molecular Probe Nanoarchitectonics for Deep RNA/DNA Illumination.ACS nano · 2026Review
- Glutamatergic Periaqueductal Gray Projections to the Locus Coeruleus Orchestrate Adaptive Arousal States in Threatening Contexts.Neuroscience bulletin · 2026Article
- Increased caecal Intestinimonas abundance inhibits E. tenella gametogenesis via EtGFAT regulation and alleviates infection through immunity.Microbiome · 2026Article
- In-situ dual-lighting system for HER2 nucleic acid and protein co-localization through simultaneous visual signal amplification in single pathological sections.Communications medicine · 2025Article
- Intratumoral microbiome: a crucial regulating factor in development and progression of colorectal cancer.Molecular biomedicine · 2025Review
- Tetrahedral DNA dendritic nanostructure-enhanced FISH for high-speed, sensitive spatial transcriptomics.Nature communications · 2025Article
- One probe fits all: a highly customizable modular RNA in situ hybridization platform expanding the application of SABER DNA probes.Development (Cambridge, England) · 2025Article
- Uncovering dynamic transcriptional regulation of methanogenesis via single-cell imaging of archaeal gene expression.Nature communications · 2025Article
- A study of repetitive sequences in the genome ofPeerJ · 2025Article
- Revolutionizing Tissue Clearing and 3-Dimensional Imaging: Transparent Embedding Solvent System for Uniform High-Resolution Imaging.BME frontiers · 2025Review
- Homebuilt Imaging-Based Spatial Transcriptomics: Tertiary Lymphoid Structures as a Case Example.Methods in molecular biology (Clifton, N.J.) · 2025Article
- Overcoming Low mRNA Expression in White Matter: A Protocol for RNA Extraction From the Optic Nerve in Large Animals for Transcriptomic Analysis.Investigative ophthalmology & visual science · 2024Article
- Coordinated cellular behavior regulated by epinephrine neurotransmitters in the nerveless placozoa.Nature communications · 2024Article
- ARTseq-FISH reveals position-dependent differences in gene expression of micropatterned mESCs.Nature communications · 2024Article
- Rapid in situ RNA imaging based on Cas12a thrusting strand displacement reaction.Nucleic acids research · 2023Article
- Oligonucleotide Fluorescence In Situ Hybridization: An Efficient Chromosome Painting Method in Plants.Plants (Basel, Switzerland) · 2023Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
18 authors at 5 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In the unprecedented single-cell sequencing and spatial multiomics era of biology, fluorescence in situ hybridization (FISH) technologies with higher sensitivity and robustness, especially for detecting short RNAs and other biomolecules, are greatly desired. Here, we develop the robust multiplex π-FISH rainbow method to detect diverse biomolecules (DNA, RNA, proteins, and neurotransmitters) individually or simultaneously with high efficiency. This versatile method is successfully applied to detect gene expression in different species, from microorganisms to plants and animals. Furthermore, we delineate the landscape of diverse neuron subclusters by decoding the spatial distribution of 21 marker genes via only two rounds of hybridization. Significantly, we combine π-FISH rainbow with hybridization chain reaction to develop π-FISH+ technology for short nucleic acid fragments, such as microRNA and prostate cancer anti-androgen therapy-resistant marker ARV7 splicing variant in circulating tumour cells from patients. Our study provides a robust biomolecule in situ detection technology for spatial multiomics investigation and clinical diagnosis.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.