ArticlePloS one2023
Cost-conscious generation of multiplexed short-read DNA libraries for whole-genome sequencing.
Article in PloS one, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed.
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- Seasonal Restructuring of Microbial Communities and Resistomes in the Shitalakshya River, Bangladesh Revealed by Shotgun Metagenomics.MicrobiologyOpen · 2026Article
- Complete genome sequencing of the fowlpox virus vaccine strain from the Livestock Research Institute, Bangladesh.Microbiology resource announcements · 2026Article
- Complete genome sequence of human papillomavirus type 45 identified from a cervical cancer patient in Bangladesh.Microbiology resource announcements · 2026Article
- Genome sequence of fowlpox virus from a field outbreak in Bangladesh.Microbiology resource announcements · 2025Article
- Complete genome sequence of a lumpy skin disease virus from a calf in the mid-northeastern region of Bangladesh.Microbiology resource announcements · 2025Article
- Abundance and transmission of antibiotic resistance and virulence genes through mobile genetic elements in integrated chicken and fish farming system.Scientific reports · 2025Article
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- An efficient multiplex approach to CRISPR/Cas9 gene editing in citrus.Plant methods · 2024Article
- Vancomycin-resistant Staphylococcus aureus (VRSA) can overcome the cost of antibiotic resistance and may threaten vancomycin's clinical durability.PLoS pathogens · 2024Article
- Plant genome evolution in the genusGenome research · 2024Article
- Chromosome-level genome of the transformable northern wattle, Acacia crassicarpa.G3 (Bethesda, Md.) · 2024Article
- Exploring the role of polymorphic interspecies structural variants in reproductive isolation and adaptive divergence in Eucalyptus.GigaScience · 2024Article
- Article
- Complete mitochondrial genomes ofMitochondrial DNA. Part B, Resources · 2024Article
- Cost-conscious generation of multiplexed short-read DNA libraries for whole-genome sequencing.PloS one · 2023Article
- The first long-read nuclear genome assembly of Oryza australiensis, a wild rice from northern Australia.Scientific reports · 2022Article
- Landscape drivers of genomic diversity and divergence in woodland Eucalyptus.Molecular ecology · 2019Article
Corrections and comments
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Massively parallel, second-generation short-read DNA sequencing has become an integral tool in biology for genomic studies. Offering highly accurate base-pair resolution at the most competitive price, the technology has become widespread. However, high-throughput generation of multiplexed DNA libraries can be costly and cumbersome. Here, we present a cost-conscious protocol for generating multiplexed short-read DNA libraries using a bead-linked transposome from Illumina. We prepare libraries in high-throughput with small reaction volumes that use 1/50th the amount of transposome compared to Illumina DNA Prep tagmentation protocols. By reducing transposome usage and optimising the protocol to circumvent magnetic bead-based clean-ups between steps, we reduce costs, labour time and DNA input requirements. Developing our own dual index primers further reduced costs and enables up to nine 96-well microplate combinations. This facilitates efficient usage of large-scale sequencing platforms, such as the Illumina NovaSeq 6000, which offers up to three terabases of sequencing per S4 flow cell. The protocol presented substantially reduces the cost per library by approximately 1/20th compared to conventional Illumina methods.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.