ArticleMolecular and cellular biology1987
Transient correction of genetic defects in cultured animal cells by introduction of functional proteins.
Article in Molecular and cellular biology, 1987. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
6 citing papers in PubMed, 11 citations in OpenAlex.
- Bead Loading Proteins and Nucleic Acids into Adherent Human Cells.Journal of visualized experiments : JoVE · 2021Article
- Gap junctions mediate bystander cell death in developing retina.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2003Article
- Dephosphorylation of tau protein by calcineurin triturated into neural living cells.Cellular and molecular neurobiology · 2002Article
- Tat-mediated delivery of heterologous proteins into cells.Proceedings of the National Academy of Sciences of the United States of America · 1994Article
- Delivery of macromolecules into living cells: a method that exploits folate receptor endocytosis.Proceedings of the National Academy of Sciences of the United States of America · 1991Article
- Bioassay for trans-activation using purified human immunodeficiency virus tat-encoded protein: trans-activation requires mRNA synthesis.Proceedings of the National Academy of Sciences of the United States of America · 1989Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Material was introduced into cultures of cells by using the method of scrape loading, in which cells are simply rubbed from the surface of a plastic tissue culture dish by a rubber-tipped rod in the presence of a macromolecule of interest. The volume of solution introduced into cells was comparable to that generally injected in the direct microinjection method with glass capillaries, that is, about 50 to 100 fl per cell. Genetic defects (lack of hypoxanthine-guanine phosphoribosyltransferase and thymidine kinase) in several cell lines were transiently corrected by scraping the cells in the presence of crude cell extracts prepared from wild-type cells.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.