ArticleNature chemical biology2023
Genome mining for unknown-unknown natural products.
Article in Nature chemical biology, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 45 papers.
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Who cites it
45 citing papers in PubMed, 98 citations in OpenAlex.
- Integrated Genome Mining and Bioactivity-Guided Isolation of Antimicrobial Peptides from Bacillus amyloliquefaciens BS4.Probiotics and antimicrobial proteins · 2026Article
- Neuroprotective Indole Diterpenoids from the FungusPharmaceuticals (Basel, Switzerland) · 2026Article
- The tRNA moieties of both aminoacyl-tRNA substrates of a cyclodipeptide synthase share a common binding site, as revealed by RNA microhelices mimicking tRNA acceptor arms.Nucleic acids research · 2026Article
- The Chemistry and Pharmacology of the Alkaloid Barettin and Its Analogues from the Marine SpongeMarine drugs · 2026Review
- Microbial unusual gene clusters without prominent core enzymes: natural products, biosynthesis and genome mining.The Journal of antibiotics · 2026Review
- Recent advances in lincosamide biosynthetic studies.The Journal of antibiotics · 2026Review
- Genome mining of unusual biosynthetic gene clusters.The Journal of antibiotics · 2026Article
- NPASS database update 2026: comprehensive quantitative composition, bioactivity, and ADME-Tox data of natural products for biomedical research.Nucleic acids research · 2026Article
- Bacterial quorum-sensing systems in cell-cell communication.FEMS microbiology reviews · 2026Review
- Biodiversity-Driven Natural Products and Bioactive Metabolites.Plants (Basel, Switzerland) · 2025Review
- Enzymatic combinatorial synthesis of E-64 and related cysteine protease inhibitors.Nature chemical biology · 2025Article
- Fungus-derived opine enhances plant photosynthesis.Journal of advanced research · 2025Article
- Microcrystal Electron Diffraction-Guided Discovery of Fungal Metabolites.Journal of the American Chemical Society · 2025Article
- Discovery of acylsulfenic acid-featuring natural product sulfenicin and characterization of its biosynthesis.Nature chemistry · 2025Article
- Genome Mining of Fungal Aza-Polycyclic Natural Products Derived from Arginine-Containing Cyclodipeptides.Journal of natural products · 2025Article
- Genome Mining of Isoindolinone-Containing Peptide Natural Products.Journal of the American Chemical Society · 2025Article
- Synthetic Biology in Natural Product Biosynthesis.Chemical reviews · 2025Review
- Metabolomic Analysis of the Responses of BryophyteInternational journal of molecular sciences · 2025Article
- How AI can help us beat AMR.npj antimicrobials and resistance · 2025Review
- In Vitro and In Silico Studies of Maculosin as a Melanogenesis and Tyrosinase Inhibitor.Molecules (Basel, Switzerland) · 2025Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
Genome mining of biosynthetic pathways with no identifiable core enzymes can lead to discovery of the so-called unknown (biosynthetic route)-unknown (molecular structure) natural products. Here we focused on a conserved fungal biosynthetic pathway that lacks a canonical core enzyme and used heterologous expression to identify the associated natural product, a highly modified cyclo-arginine-tyrosine dipeptide. Biochemical characterization of the pathway led to identification of a new arginine-containing cyclodipeptide synthase (RCDPS), which was previously annotated as a hypothetical protein and has no sequence homology to non-ribosomal peptide synthetase or bacterial cyclodipeptide synthase. RCDPS homologs are widely encoded in fungal genomes; other members of this family can synthesize diverse cyclo-arginine-Xaa dipeptides, and characterization of a cyclo-arginine-tryptophan RCDPS showed that the enzyme is aminoacyl-tRNA dependent. Further characterization of the biosynthetic pathway led to discovery of new compounds whose structures would not have been predicted without knowledge of RCDPS function.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.