Evidence map›Paper›PMID 36702897›Full record

ArticleNature biotechnology2023

Three-dimensional structured illumination microscopy with enhanced axial resolution.

Xuesong Li, Yicong Wu, Yijun Su, Ivan Rey-Suarez, Claudia Matthaeus, Taylor B Updegrove, Zhuang Wei, Lixia Zhang, Hideki Sasaki, Yue Li and 12 more

Open access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Nature biotechnology, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 44 papers.

0numbers the graph read from it
0cells of the map it votes in
44citing papers in PubMed
39.6field-weighted citation impact, top 1% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

44 citing papers in PubMed, 112 citations in OpenAlex.

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  14. Case Study: Illuminating the Nanoscale World of Microbiology.Methods in molecular biology (Clifton, N.J.) · 2026
    Review
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  19. The CENP-A chaperone complex spatially organizes centromeres.bioRxiv : the preprint server for biology · 2025
    Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

22 authors at 6 institutions in 2 countries.

Xuesong LiLaboratory of High Resolution Optical Imaging, National Institute of Biomedical Imaging and Bioengineering, National Institutes of Health, Bethesda, MD, USA. lix3@janelia.hhmi.org.ORCID http://orcid.org/0000-0002-1795-4885
Yicong WuLaboratory of High Resolution Optical Imaging, National Institute of Biomedical Imaging and Bioengineering, National Institutes of Health, Bethesda, MD, USA. yicong.wu@nih.gov.ORCID http://orcid.org/0000-0002-8819-7527
Yijun SuLaboratory of High Resolution Optical Imaging, National Institute of Biomedical Imaging and Bioengineering, National Institutes of Health, Bethesda, MD, USA.
Ivan Rey-SuarezInstitute for Physical Science and Technology, University of Maryland, College Park, MD, USA.
Claudia MatthaeusBiochemistry and Biophysics Center, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD, USA.
Taylor B UpdegroveLaboratory of Molecular Biology, National Cancer Institute, National Institutes of Health, Bethesda, MD, USA.
Zhuang WeiSection on Biophotonics, National Institute of Biomedical Imaging and Bioengineering, National Institutes of Health, Bethesda, MD, USA.
Lixia ZhangAdvanced Imaging and Microscopy Resource, National Institutes of Health, Bethesda, MD, USA.
Hideki SasakiLeica Microsystems, Inc., Deerfield, IL, USA.ORCID http://orcid.org/0000-0002-5926-3740
Yue LiState Key Laboratory of Modern Optical Instrumentation, College of Optical Science and Engineering, Zhejiang University, Hangzhou, China.ORCID http://orcid.org/0000-0002-9299-0974
Min GuoLaboratory of High Resolution Optical Imaging, National Institute of Biomedical Imaging and Bioengineering, National Institutes of Health, Bethesda, MD, USA.ORCID http://orcid.org/0000-0002-2093-8771
John P GianniniLaboratory of High Resolution Optical Imaging, National Institute of Biomedical Imaging and Bioengineering, National Institutes of Health, Bethesda, MD, USA.
Harshad D VishwasraoAdvanced Imaging and Microscopy Resource, National Institutes of Health, Bethesda, MD, USA.
Jiji ChenAdvanced Imaging and Microscopy Resource, National Institutes of Health, Bethesda, MD, USA.ORCID http://orcid.org/0000-0002-4426-3035
Shih-Jong J LeeLeica Microsystems, Inc., Deerfield, IL, USA.
Lin ShaoDepartment of Neuroscience and Department of Cell Biology, Yale University School of Medicine, New Haven, CT, USA.
Huafeng LiuState Key Laboratory of Modern Optical Instrumentation, College of Optical Science and Engineering, Zhejiang University, Hangzhou, China.
Kumaran S RamamurthiLaboratory of Molecular Biology, National Cancer Institute, National Institutes of Health, Bethesda, MD, USA.
Justin W TaraskaBiochemistry and Biophysics Center, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD, USA.ORCID http://orcid.org/0000-0001-5355-9535
Arpita UpadhyayaInstitute for Physical Science and Technology, University of Maryland, College Park, MD, USA.
Patrick La RiviereDepartment of Radiology, University of Chicago, Chicago, IL, USA.ORCID http://orcid.org/0000-0003-3415-9864
Hari ShroffLaboratory of High Resolution Optical Imaging, National Institute of Biomedical Imaging and Bioengineering, National Institutes of Health, Bethesda, MD, USA.
National Institutes of Health · USBellevue College · USState Key Laboratory of Modern Optical InstrumentsUniversity of Maryland, College Park · USMarine Biological Laboratory · USYale University · US

Funding

Architecture and control of vesicle fusion in excitable cellsZIAHL006098 · NHLBI · NATIONAL HEART, LUNG, AND BLOOD INSTITUTE · PI TARASKA, JUSTIN · 2011 to 2025
$25.5M
Subcellular protein localization in B. subtilisZIABC011211 · NCI · DIVISION OF BASIC SCIENCES - NCI · PI RAMAMURTHI, KUMARAN · 2009 to 2025
$21.4M
Supplement request for Microtubule regulation of actomyosin dynamics and force generation in T lymphocytesR01GM131054 · NIGMS · UNIV OF MARYLAND, COLLEGE PARK · PI UPADHYAYA, ARPITA · 2019 to 2022
$1.4M
Howard Hughes Medical InstituteNIGMS NIH HHS R01 GM131054
6 · The paper itself

Abstract

The axial resolution of three-dimensional structured illumination microscopy (3D SIM) is limited to ∼300 nm. Here we present two distinct, complementary methods to improve axial resolution in 3D SIM with minimal or no modification to the optical system. We show that placing a mirror directly opposite the sample enables four-beam interference with higher spatial frequency content than 3D SIM illumination, offering near-isotropic imaging with ∼120-nm lateral and 160-nm axial resolution. We also developed a deep learning method achieving ∼120-nm isotropic resolution. This method can be combined with denoising to facilitate volumetric imaging spanning dozens of timepoints. We demonstrate the potential of these advances by imaging a variety of cellular samples, delineating the nanoscale distribution of vimentin and microtubule filaments, observing the relative positions of caveolar coat proteins and lysosomal markers and visualizing cytoskeletal dynamics within T cells in the early stages of immune synapse formation.

Indexed as

Imaging, Three-DimensionalLightingCytoskeletonLysosomesMicroscopy, Fluorescence

Identifiers

PMID36702897
PMCPMC10497409
OpenAlexW4318071492

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.