ArticleNature communications2023
Evolution of giant pandoravirus revealed by CRISPR/Cas9.
Article in Nature communications, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed.
- Infection cycles of viruses of the phylum Nucleocytoviricota.Nature reviews. Microbiology · 2026Review
- Why did some viruses evolve to be giants while others did not?Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- Horizontal transfer of a 180-kbp genomic fraction among the largest viral genomes.Applied and environmental microbiology · 2026Article
- Refining a giant virus lineage: a novel order unifyingJournal of virology · 2026Article
- Rise of the viral giants: common themes underlying genome gigantism in eukaryotic viruses and bacteriophages.Current opinion in microbiology · 2026Review
- Elucidating the protein interaction network of one of the largest icosahedral capsids in the virosphere.The EMBO journal · 2026Article
- The dual-origin hypothesis of viruses under environmental stress and energy crisis.Theory in biosciences = Theorie in den Biowissenschaften · 2026Article
- Genome-wide Characterization of Non-shared Sequences among Amphora-shaped Giant Viruses.Microbes and environments · 2026Article
- The proteomics of the giant cedratvirus particles reveals unique and shared features with pitho-like viruses.Journal of virology · 2025Article
- Evolutionarily conserved grammar rules viral factories of amoeba-infecting members of the hyperdiverseProceedings of the National Academy of Sciences of the United States of America · 2025Article
- Melbournevirus encodes a shorter H2B-H2A doublet histone variant that forms structurally distinct nucleosome structures.Nature communications · 2025Article
- 20 years of research on giant viruses.Npj viruses · 2025Review
- Complex transcriptional regulations of a hyperparasitic quadripartite system in giant viruses infecting protists.Nature communications · 2024Article
- On the evolutionary developmental biology of the cell.Trends in genetics : TIG · 2024Review
- Adaptive Evolution Signatures inMicroorganisms · 2024Article
- The consequences of viral infection on protists.Communications biology · 2024Review
- Genetic manipulation of giant viruses and their host, Acanthamoeba castellanii.Nature protocols · 2024Review
- Cytopathic effects in Mimivirus infection: understanding the kinetics of virus-cell interaction.Memorias do Instituto Oswaldo Cruz · 2024Article
- Functional redundancy revealed by the deletion of the mimivirus GMC-oxidoreductase genes.microLife · 2024Article
- Gene duplication as a major force driving the genome expansion in some giant viruses.Journal of virology · 2023Article
Corrections and comments
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Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Giant viruses (GVs) are a hotspot of unresolved controversies since their discovery, including the definition of "Virus" and their origin. While increasing knowledge of genome diversity has accumulated, GV functional genomics was largely neglected. Here, we describe an experimental framework to genetically modify nuclear GVs and their host Acanthamoeba castellanii using CRISPR/Cas9, shedding light on the evolution from small icosahedral viruses to amphora-shaped GVs. Ablation of the icosahedral major capsid protein in the phylogenetically-related mollivirus highlights a transition in virion shape and size. We additionally demonstrate the existence of a reduced core essential genome in pandoravirus, reminiscent of their proposed smaller ancestors. This proposed genetic expansion led to increased genome robustness, indicating selective pressures for adaptation to uncertain environments. Overall, we introduce new tools for manipulation of the unexplored genome of nuclear GVs and provide experimental evidence suggesting that viral gigantism has aroused as an emerging trait.
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