ArticlePLoS genetics2023
Shuffling the yeast genome using CRISPR/Cas9-generated DSBs that target the transposable Ty1 elements.
Article in PLoS genetics, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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21 citing papers in PubMed, 21 citations in OpenAlex.
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- Expression of EndonucleaseCurrent issues in molecular biology · 2026Article
- A CRISPR-Cas9-based system for the dose-dependent study of DNA double-strand break sensing and repair.The FEBS journal · 2025Article
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- Fueling chromosomal gene diversification and artificial evolution with CRISPR.Genome biology · 2025Review
- High-quality genome assembly and comparative analysis reveal extensive genomic variation inMicrobial genomics · 2025Article
- A comparative genomic analysis at the chromosomal-level reveals evolutionary patterns of aphid chromosomes.Communications biology · 2025Article
- Spontaneous and environment induced genomic alterations in yeast model.Cell insight · 2025Review
- Patterns of spontaneous and induced genomic alterations inApplied and environmental microbiology · 2025Article
- Genomic characteristics and genetic manipulation of the marine yeast Scheffersomyces spartinae.Applied microbiology and biotechnology · 2024Article
- Exploration of In Situ Extraction for Enhanced Triterpenoid Production by Saccharomyces cerevisiae.Microbial biotechnology · 2024Article
- Peteryoungia algae sp. nov. isolated from seaweeds of Gouqi Island, China, and its unique genetic features among Peteryoungia strains.Antonie van Leeuwenhoek · 2024Article
- Transposon Removal Reveals Their Adaptive Fitness Contribution.Genome biology and evolution · 2024Article
- Large-scale genomic rearrangements boost SCRaMbLE in Saccharomyces cerevisiae.Nature communications · 2024Article
- Splitting the yeast centromere by recombination.Nucleic acids research · 2024Article
- Nonlethal Furfural Exposure Causes Genomic Alterations and Adaptability Evolution in Saccharomyces cerevisiae.Microbiology spectrum · 2023Article
- Practical Approaches for the YeastInternational journal of molecular sciences · 2023Review
- Delineation of two multi-invasion-induced rearrangement pathways that differently affect genome stability.Genes & development · 2023Article
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10 authors at 4 institutions in 2 countries.
Funding
Abstract
Although homologous recombination between transposable elements can drive genomic evolution in yeast by facilitating chromosomal rearrangements, the details of the underlying mechanisms are not fully clarified. In the genome of the yeast Saccharomyces cerevisiae, the most common class of transposon is the retrotransposon Ty1. Here, we explored how Cas9-induced double-strand breaks (DSBs) directed to Ty1 elements produce genomic alterations in this yeast species. Following Cas9 induction, we observed a significant elevation of chromosome rearrangements such as deletions, duplications and translocations. In addition, we found elevated rates of mitotic recombination, resulting in loss of heterozygosity. Using Southern analysis coupled with short- and long-read DNA sequencing, we revealed important features of recombination induced in retrotransposons. Almost all of the chromosomal rearrangements reflect the repair of DSBs at Ty1 elements by non-allelic homologous recombination; clustered Ty elements were hotspots for chromosome rearrangements. In contrast, a large proportion (about three-fourths) of the allelic mitotic recombination events have breakpoints in unique sequences. Our analysis suggests that some of the latter events reflect extensive processing of the broken ends produced in the Ty element that extend into unique sequences resulting in break-induced replication. Finally, we found that haploid and diploid strain have different preferences for the pathways used to repair double-stranded DNA breaks. Our findings demonstrate the importance of DNA lesions in retrotransposons in driving genome evolution.
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