Evidence map›Paper›PMID 36700708›Full record

ArticleParasite (Paris, France)2023

Real-time multiplex PCR for human echinococcosis and differential diagnosis.

Jenny Knapp, Séverine Lallemand, Franck Monnien, Sophie Felix, Sandra Courquet, Gérald Umhang, Laurence Millon

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Parasite (Paris, France), 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
11citing papers in PubMed, 1 pooled it
4.6field-weighted citation impact, top 5% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

11 citing papers in PubMed, 1 synthesis or guideline pooled it, 18 citations in OpenAlex.

  1. Pooled it
  2. Review
  3. Article
  4. Review
  5. Article
  6. Article
  7. EchiNam: multicenter retrospective study on the experience, challenges, and pitfalls in the diagnosis and treatment of alveolar echinococcosis in Belgium.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2025
    Article
  8. Article
  9. Review
  10. Review
  11. High species diversity ofInternational journal for parasitology. Parasites and wildlife · 2023
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 3 institutions in 1 country.

Jenny KnappDepartment of Parasitology-Mycology, National Reference Centre for Echinococcoses, University Hospital of Besançon, 25030 Besançon, France - UMR CNRS 6249 Laboratoire Chrono-environnement, University of Franche-Comté, 16 Route de Gray, 25030 Besançon, France.
Séverine LallemandUMR CNRS 6249 Laboratoire Chrono-environnement, University of Franche-Comté, 16 Route de Gray, 25030 Besançon, France.
Franck MonnienDepartment of Pathology, University Hospital of Besançon, 25030 Besançon, France.
Sophie FelixDepartment of Pathology, University Hospital of Besançon, 25030 Besançon, France.
Sandra CourquetDepartment of Parasitology-Mycology, National Reference Centre for Echinococcoses, University Hospital of Besançon, 25030 Besançon, France - UMR CNRS 6249 Laboratoire Chrono-environnement, University of Franche-Comté, 16 Route de Gray, 25030 Besançon, France.
Gérald UmhangANSES Nancy laboratory for Rabies and Wildlife, National Reference Laboratory for Echinococcus spp., Wildlife Surveillance and Eco-epidemiology Unit, Technopole Agricole et Vétérinaire, 54220 Malzéville, France.
Laurence MillonDepartment of Parasitology-Mycology, National Reference Centre for Echinococcoses, University Hospital of Besançon, 25030 Besançon, France - UMR CNRS 6249 Laboratoire Chrono-environnement, University of Franche-Comté, 16 Route de Gray, 25030 Besançon, France.
Centre National de la Recherche Scientifique · FRCentre Hospitalier Universitaire de Besançon · FROffice National de la Chasse et de la Faune Sauvage · FR

Funding

CNR Echinococcoses
6 · The paper itself

Abstract

Molecular identification of rare human infectious pathogens appears to be one of the most relevant current methods for rapid diagnosis and management of patients. PCR techniques, in particular real-time quantitative PCR, are best suited for the detection of DNA from the pathogens, even at low concentrations. Echinococcosis infections are due to helminths of the Echinococcus genus, with closely related species involved in parasitic lesions affecting animals and, accidentally, humans. We developed a multiplex qPCR (MLX qPCR) assay allowing for the detection of four Echinococcus species involved in Europe in alveolar echinococcosis (AE) and cystic echinococcosis (CE) (Echinococcus multilocularis, E. granulosus sensu stricto, E. ortleppi, and E. canadensis), based on short mitochondrial targets. A collection of 81 fresh and formalin-fixed paraffin-embedded tissues (FFPE) of AE and CE lesions was assembled. The qPCR assays were performed in triplex for Echinococcus spp. detection, associated with a qPCR inhibitor control. A duplex qPCR was also designed to enable diagnosis of two other dead-end helminthiases (cysticercosis (Taenia solium), and toxocariasis (Toxocara cati and T. canis)). The sensitivity of the qPCR was assessed and ranged from 1 to 5 × 10

Indexed as

EchinococcosisEchinococcus granulosusEchinococcus multilocularisAnimalsDiagnosis, DifferentialHumansMultiplex Polymerase Chain ReactionFFPEFresh materialHuman echinococcosisMolecular diagnosisMultiplexingQuantitative real-time PCR

Identifiers

PMID36700708
PMCPMC9886084
OpenAlexW4318066316

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.