ArticleBioinformatics advances2021
PoET: automated approach for measuring pore edge tension in giant unilamellar vesicles.
Article in Bioinformatics advances, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed.
- Electric field-induced rupture kinetics of giant unilamellar vesicles with varying gramicidin A content in membrane.PloS one · 2026Article
- Interaction of anionic FeRSC advances · 2024Review
- Bilayer Charge Asymmetry and Oil Residues Destabilize Membranes upon Poration.Langmuir : the ACS journal of surfaces and colloids · 2024Article
- DisGUVery: A Versatile Open-Source Software for High-Throughput Image Analysis of Giant Unilamellar Vesicles.ACS synthetic biology · 2023Article
- Article
- Assessing membrane material properties from the response of giant unilamellar vesicles to electric fields.Advances in physics: X · 2023Article
- Complexation of a Polypeptide-Polyelectrolytes Bioparticle as a Biomaterial of Antibacterial Activity.Pharmaceutics · 2022Article
- Article
- GM1 asymmetry in the membrane stabilizes pores.Biophysical journal · 2022Article
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Authors and funding
4 authors.
Funding
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Abstract
Motivation: A reliable characterization of the membrane pore edge tension of single giant unilamellar vesicles (GUVs) requires the measurement of micrometer sized pores in hundreds to thousands of images. When manually performed, this procedure has shown to be extremely time-consuming and to generate inconsistent results among different users and imaging systems. A user-friendly software for such analysis allowing quick processing and generation of reproducible data had not yet been reported. Results: We have developed a software (PoET) for automatic pore edge tension measurements on GUVs. The required image processing steps and the characterization of the pore dynamics are performed automatically within the software and its use allowed for a 30-fold reduction in the analysis time. We demonstrate the applicability of the software by comparing the pore edge tension of GUVs of different membrane compositions and surface charges. The approach was applied to electroporated GUVs but is applicable to other means of pore formation. Availability and implementation: The complete software is implemented in Python and available for Windows at https://dx.doi.org/10.17617/3.7h. Supplementary information: Supplementary data are available at
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Registered trials
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