ArticleBMC plant biology2023
Direct leaf-peeling method for areca protoplasts: a simple and efficient system for protoplast isolation and transformation in areca palm (Areca catechu).
Article in BMC plant biology, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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Who cites it
8 citing papers in PubMed, 12 citations in OpenAlex.
- Research Progress on Preparation, Transformation and Application of Protoplasts Derived from Medicinal Plants.Plants (Basel, Switzerland) · 2026Review
- Protoplast isolation and transient gene expression in Suaeda aralocaspica, a halophyte with single-cell CPlant methods · 2026Article
- The Impact of Reduced Nitrogen Fertilizer Application andPlants (Basel, Switzerland) · 2025Article
- Development of Protoplast-Based Gene Editing System for Areca Palm.Plants (Basel, Switzerland) · 2025Article
- Identification of a novel mild isolate of areca palm necrotic spindle-spot virus (ANSSVm) lacking two cysteine proteases (HC-Pro1 and HC-Pro2).Frontiers in microbiology · 2025Article
- Optimization of preparation and transformation of protoplasts from Populus simonii × P. nigra leaves and subcellular localization of the major latex protein 328 (MLP328).Plant methods · 2024Article
- Isolation, Purification, and Application of Protoplasts and Transient Expression Systems in Plants.International journal of molecular sciences · 2023Review
- Cotyledon peeling method for passion fruit protoplasts: a versatile cell system for transient gene expression in passion fruitFrontiers in plant science · 2023Article
Corrections and comments
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Authors and funding
10 authors at 3 institutions in 2 countries.
Funding
Abstract
backgroundAreca palm (Areca catechu) is a woody perennial plant of both economical and medicinal importance grown in tropical and subtropical climates. Yet, the molecular biology study of areca palm is extremely impeded by its unavailability of a transformation method. An efficient protoplast isolation and transformation system could be highly desirable to overcome this barrier.
resultsHere, we described a simple and efficient method for protoplast isolation and transformation from the perennial plant areca palm. A high yield of protoplasts (2.5 × 10
conclusionWe have established the first efficient protocol for the high-yield isolation and transformation of areca palm protoplasts. This method shall be applied in various biological studies of areca palm, such as gene function analysis, genome editing, protein trafficking and localization and protein-protein interaction. In addition, the protoplast system offers a great genetic transformation approach for the woody perennial plant-areca palm. Moreover, the established platform may be applied in protoplast isolation and transformation for other important species in the palm family, including oil palm and coconut.
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