ArticleCommunications chemistry2021
Surfactant-free production of biomimetic giant unilamellar vesicles using PDMS-based microfluidics.
Article in Communications chemistry, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
37 citing papers in PubMed, 68 citations in OpenAlex.
- Uniform Microscale Thermoresponsive Liposomes for Controlled Release Above Body Temperature.Small (Weinheim an der Bergstrasse, Germany) · 2026Article
- Membrane-Associated Biomolecules for Synthetic Cell Signalling.Chembiochem : a European journal of chemical biology · 2026Review
- Systematic investigation of double emulsion dewetting dynamics for the robust production of giant unilamellar vesicles.Lab on a chip · 2026Article
- A robust method for on-chip production and manipulation of lipid vesicles by inverted emulsion.Cell reports methods · 2026Article
- Engineering synthetic cells with intramembrane domains possessing distinct bilayer asymmetries.Nature communications · 2026Article
- Design of a Trigger-Responsive Photothermal Vesicle-Based Cargo Delivery Platform.Small (Weinheim an der Bergstrasse, Germany) · 2025Article
- Artificial cells with liquid-liquid phase separation-regulated cell-free protein synthesis.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Lipid-Mediated Assembly of Biomolecular Condensates: Mechanisms, Regulation, and Therapeutic Implications.Biology · 2025Review
- Innovative composite systems for enhancing plant polyphenol stability and bioavailability.Food science and biotechnology · 2025Review
- Low-Cost, Open-Source, High-Precision Pressure Controller for Multi-Channel Microfluidics.Biosensors · 2025Article
- Rapid Multi-Well Evaluation of Assorted Materials for Hydrogel-Assisted Giant Unilamellar Vesicle Production: Empowering Bottom-Up Synthetic Biology.Gels (Basel, Switzerland) · 2025Article
- Challenges in observing transcription-translation for bottom-up synthetic biology.QRB discovery · 2025Review
- Facile and versatile PDMS-glass capillary double emulsion formation device coupled with rapid purification toward microfluidic giant liposome generation.Microsystems & nanoengineering · 2024Article
- Droplet Microfluidics for High-Throughput Screening and Directed Evolution of Biomolecules.Micromachines · 2024Review
- Emerging Designs and Applications for Biomembrane Biosensors.Annual review of analytical chemistry (Palo Alto, Calif.) · 2024Review
- Microfluidic production, stability and loading of synthetic giant unilamellar vesicles.Scientific reports · 2024Article
- Complex Emulsions as an Innovative Pharmaceutical Dosage form in Addressing the Issues of Multi-Drug Therapy and Polypharmacy Challenges.Pharmaceutics · 2024Review
- Glass Microdroplet Generator for Lipid-Based Double Emulsion Production.Micromachines · 2024Article
- Formation of Giant Unilamellar Vesicles Assisted by Fluorinated Nanoparticles.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2023Article
- Preparation and biomedical applications of artificial cells.Materials today. Bio · 2023Review
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
Microfluidic production of giant lipid vesicles presents a paradigm-shift in the development of artificial cells. While production is high-throughput and the lipid vesicles are mono-disperse compared to bulk methods, current technologies rely heavily on the addition of additives such as surfactants, glycerol and even ethanol. Here we present a microfluidic method for producing biomimetic surfactant-free and additive-free giant unilamellar vesicles. The versatile design allows for the production of vesicle sizes ranging anywhere from ~10 to 130 µm with either neutral or charged lipids, and in physiological buffer conditions. Purity, functionality, and stability of the membranes are validated by lipid diffusion, protein incorporation, and leakage assays. Usability as artificial cells is demonstrated by increasing their complexity, i.e., by encapsulating plasmids, smaller liposomes, mammalian cells, and microspheres. This robust method capable of creating truly biomimetic artificial cells in high-throughput will prove valuable for bottom-up synthetic biology and the understanding of membrane function.
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.