Evidence map›Paper›PMID 36696043›Full record

ArticleInfection2023

Prospective flow cytometry analysis of leucocyte subsets in critically ill patients who develop sepsis: a pilot study.

Nathalie Layios, Christian Gosset, Nathalie Maes, Céline Delierneux, Alexandre Hego, Justine Huart, Christelle Lecut, Pierre Damas, Cécile Oury, André Gothot

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Article in Infection, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
0.2field-weighted citation impact, top 47% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 1 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 2 institutions in 1 country.

Nathalie LayiosDepartment of Intensive Care, University Hospital of Liege, Domaine universitaire du Sart-Tilman, 4000, Liege, Belgium. Nathalie.layios@chuliege.be.ORCID http://orcid.org/0000-0002-1360-4917
Christian GossetDepartment of Hematobiology and Immuno-Hematology, University Hospital of Liege, Liege, Belgium.
Nathalie MaesBiostatistics and Research Method Center, University Hospital of Liege, Liege, Belgium.
Céline DelierneuxLaboratory of Cardiology, GIGA Institute, University Hospital of Liege, Liege, Belgium.
Alexandre HegoLaboratory of Thrombosis and Hemostasis, GIGA-Cardiovascular Sciences, University of Liege, Liege, Belgium.
Justine HuartDepartment of Nephrology, University Hospital of Liege, Liege, Belgium.
Christelle LecutDepartment of Hematobiology and Immuno-Hematology, University Hospital of Liege, Liege, Belgium.
Pierre DamasDepartment of Intensive Care, University Hospital of Liege, Domaine universitaire du Sart-Tilman, 4000, Liege, Belgium.
Cécile OuryLaboratory of Cardiology, GIGA Institute, University Hospital of Liege, Liege, Belgium.
André GothotDepartment of Hematobiology and Immuno-Hematology, University Hospital of Liege, Liege, Belgium.
University of Liège · BECentre Hospitalier Universitaire de Liège · BE

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

purposeSepsis in critically ill patients with injury bears a high morbidity and mortality. Extensive phenotypic monitoring of leucocyte subsets in critically ill patients at ICU admission and during sepsis development is still scarce. The main objective of this study was to identify early changes in leukocyte phenotype which would correlate with later development of sepsis.

methodsPatients who were admitted in a tertiary ICU for organ support after severe injury (elective cardiac surgery, trauma, necessity of prolonged ventilation or stroke) were sampled on admission (T1) and 48-72 h later (T2) for phenotyping of leukocyte subsets by flow cytometry and cytokines measurements. Those who developed secondary sepsis or septic shock were sampled again on the day of sepsis diagnosis (Tx).

resultsNinety-nine patients were included in the final analysis. Nineteen (19.2%) patients developed secondary sepsis or septic shock. They presented significantly higher absolute monocyte counts and CRP at T1 compared to non-septic patients (1030/µl versus 550/µl, p = 0.013 and 5.1 mg/ml versus 2.5 mg/ml, p = 0.046, respectively). They also presented elevated levels of monocytes with low expression of L-selectin (CD62L

conclusionMonocyte counts and change of phenotype are associated with secondary sepsis occurrence in critically ill patients with injury.

Indexed as

SepsisShock, SepticCritical IllnessFlow CytometryHLA-DR AntigensHumansMonocytesPilot ProjectsProspective StudiesHLA-DR AntigensFlow cytometryHLA-DRInjuryL-selectinMonocytesSepsis

Identifiers

PMID36696043
OpenAlexW4317932561

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.