ArticlePlant cell reports2023
GRF-GIF chimeric proteins enhance in vitro regeneration and Agrobacterium-mediated transformation efficiencies of lettuce (Lactuca spp.).
Article in Plant cell reports, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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Who cites it
32 citing papers in PubMed, 44 citations in OpenAlex.
- Chimeras of GROWTH-REGULATING FACTOR and GRF-INTERACTING FACTOR enhance leaf-based regeneration and transformation efficiency in tomato.Journal of experimental botany · 2026Article
- Plant Regeneration: Influencing Factors, Regulatory Networks, and Epigenetic Mechanisms.International journal of molecular sciences · 2026Review
- Efficient Embryogenic Callus Induction andPlants (Basel, Switzerland) · 2026Article
- Mammalian growth factors enhance regeneration in transgenic tomato lines.Transgenic research · 2026Article
- WUSCHEL in forest trees: evolutionary organizer, vascular architect, and totipotency switch.Plant molecular biology · 2026Review
- A Rapid Hairy Root-Based Platform for CRISPR/Cas Optimization and Guide RNA Validation in Lettuce.Plants (Basel, Switzerland) · 2026Article
- Genome-wide identification of the GRF gene family and response to cold stress in astragalus (Astragalus membranaceus).BMC genomics · 2026Article
- Genome-Wide Identification and Expression Analysis of theInternational journal of molecular sciences · 2026Article
- Molecular Regulators of In Vitro Regeneration in Wheat: Roles of Morphogenic Factors in Transformation, Genome Editing, and Breeding.International journal of molecular sciences · 2026Review
- Establishment of an efficientFrontiers in plant science · 2026Article
- Advances in the use of morphogenic regulators and peptide regenerating factors for boosting plant transformation and genome editing.Frontiers in plant science · 2026Review
- Auxin affects gene editing efficiency through regulating chromatin accessibility and plant regeneration process.Horticulture research · 2025Article
- Monitoring the stability of transgene expression in lettuce using the RUBY reporter.Plant cell reports · 2025Article
- Rational design of induced regeneration via somatic embryogenesis in the absence of exogenous phytohormones.The Plant cell · 2025Article
- Mechanism and Application of Developmental Factors in Plant Genetic Transformation.International journal of molecular sciences · 2025Review
- Application of a GRF-GIF chimera enhances plant regeneration for genome editing in tomato.Plant biotechnology journal · 2025Article
- Conquering Limitations: Exploring the Factors that Drive Successful Agrobacterium-Mediated Genetic Transformation of Recalcitrant Plant Species.Molecular biotechnology · 2025Review
- Plant genetic transformation: achievements, current status and future prospects.Plant biotechnology journal · 2025Review
- MicroRNA control of stem cell reconstitution and growth in root regeneration.Nature plants · 2025Article
- Agrobacterium-mediated Cuscuta campestris transformation as a tool for understanding plant-plant interactions.The New phytologist · 2025Article
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
Abstract
key messageGRF-GIF chimeric proteins from multiple source species enhance in vitro regeneration in both wild and cultivated lettuce. In addition, they enhance regeneration in multiple types of lettuce including butterheads, romaines, and crispheads. The ability of plants to regenerate in vitro has been exploited for use in tissue culture systems for plant propagation, plant transformation, and genome editing. The success of in vitro regeneration is often genotype dependent and continues to be a bottleneck for Agrobacterium-mediated transformation and its deployment for improvement of some crop species. Manipulation of transcription factors that play key roles in plant development such as BABY BOOM, WUSCHEL, and GROWTH-REGULATING FACTORs (GRFs) has improved regeneration and transformation efficiencies in several plant species. Here, we compare the efficacy of GRF-GIF gene fusions from multiple species to boost regeneration efficiency and shooting frequency in four genotypes of wild and cultivated lettuce (Lactuca spp. L.). In addition, we show that GRF-GIFs with mutated miRNA 396 binding sites increase regeneration efficiency and shooting frequency when compared to controls. We also present a co-transformation strategy for increased transformation efficiency and recovery of transgenic plants harboring a gene of interest. This strategy will enhance the recovery of transgenic plants of other lettuce genotypes and likely other crops in the Compositae family.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.