Evidence map›Paper›PMID 36693101›Full record

ArticleProceedings of the National Academy of Sciences of the United States of America2023

In vivo expression vector derived from anhydrobiotic tardigrade genome enables live imaging in Eutardigrada.

Sae Tanaka, Kazuhiro Aoki, Kazuharu Arakawa

Open access · greenAbstract read
In one paragraph

Article in Proceedings of the National Academy of Sciences of the United States of America, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.

0numbers the graph read from it
0cells of the map it votes in
16citing papers in PubMed
33.3field-weighted citation impact, top 1% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

16 citing papers in PubMed, 41 citations in OpenAlex.

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  14. Reply to Tanaka and Kunieda: Control protein GFP also shows a mesh-like structure in desiccating tardigrade cells.Proceedings of the National Academy of Sciences of the United States of America · 2023
    Article
  15. Nondomain biopolymers: Flexible molecular strategies to acquire biological functions.Genes to cells : devoted to molecular & cellular mechanisms · 2023
    Review
  16. The Embryonic Origin of Primordial Germ Cells in the TardigradebioRxiv : the preprint server for biology · 2023
    Article
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

3 authors at 2 institutions in 1 country.

Sae TanakaInstitute for Advanced Biosciences, Keio University, Tsuruoka, 997-0017, Japan.ORCID 0000-0002-3460-2777
Kazuhiro AokiExploratory Research Center on Life and Living Systems, National Institutes of Natural Sciences, Okazaki, 444-8787, Japan.ORCID 0000-0001-7263-1555
Kazuharu ArakawaInstitute for Advanced Biosciences, Keio University, Tsuruoka, 997-0017, Japan.ORCID 0000-0002-2893-4919
National Institutes of Natural Sciences · JPNational Institute for Basic Biology · JP

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Water is essential for life, but anhydrobiotic tardigrades can survive almost complete dehydration. Anhydrobiosis has been a biological enigma for more than a century with respect to how organisms sustain life without water, but the few choices of genetic toolkits available in tardigrade research have been a challenging circumstance. Here, we report the development of an in vivo expression system for tardigrades. This transient transgenic technique is based on a plasmid vector (TardiVec) with promoters that originated from an anhydrobiotic tardigrade

Indexed as

Intrinsically Disordered ProteinsTardigradaAnimalsDesiccationWaterIntrinsically Disordered ProteinsWateranhydrobiosisin vivo expressionlive imagingtardigrades

Identifiers

PMID36693101
PMCPMC9945988
OpenAlexW4317851265

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.