Evidence map›Paper›PMID 36692661›Full record

ArticleMolecular imaging and biology2023

Development and Biodistribution of a Nerve Growth Factor Radioactive Conjugate for PET Imaging.

R A Carrasco, A K Salih, M Dominguez Garcia, E S Khozeimeh, G P Adams, C P Phenix, E W Price

Abstract read
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In one paragraph

Article in Molecular imaging and biology, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact, top 99% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 0 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 1 institution in 1 country.

R A CarrascoDepartment of Chemistry, College of Arts and Science, University of Saskatchewan, 110 Science Place, Saskatoon, SK, S7N5C9, Canada.
A K SalihDepartment of Chemistry, College of Arts and Science, University of Saskatchewan, 110 Science Place, Saskatoon, SK, S7N5C9, Canada.
M Dominguez GarciaDepartment of Chemistry, College of Arts and Science, University of Saskatchewan, 110 Science Place, Saskatoon, SK, S7N5C9, Canada.
E S KhozeimehDepartment of Chemistry, College of Arts and Science, University of Saskatchewan, 110 Science Place, Saskatoon, SK, S7N5C9, Canada.
G P AdamsDepartment of Veterinary Biomedical Sciences, Western College of Veterinary Medicine, University of Saskatchewan, 52 Campus Drive, Saskatoon, SK, S7N5B4, Canada.
C P PhenixDepartment of Chemistry, College of Arts and Science, University of Saskatchewan, 110 Science Place, Saskatoon, SK, S7N5C9, Canada. chris.phenix@usask.ca.
E W PriceDepartment of Chemistry, College of Arts and Science, University of Saskatchewan, 110 Science Place, Saskatoon, SK, S7N5C9, Canada. eric.price@usask.ca.ORCID 0000-0002-8619-1199
University of Saskatchewan · CA

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

purposeThe purpose of these studies was to develop a nerve growth factor (NGF) radiometal-chelator conjugate to determine the biodistribution and brain uptake of NGF by positron emission tomography/computerized tomography (PET-CT). PROCEDURES: Purified NGF from llama seminal plasma was conjugated with FITC, and the chelator NOTA or DFO. NGF conjugates were evaluated for bioactivity. NOTA- and DFO-conjugated NGF were radiolabeled with gallium-68 or zirconium-89 ([

resultsCell-based in vitro assays confirmed that the NGF conjugates maintained NGF receptor-binding and biological activity. Zirconium-89 and gallium-68 radiolabeling showed a high efficiency; however, only[

conclusionsWe conclude that conjugation of DFO to NGF through a thiourea linkage allows effective radiolabeling with zirconium-89 while maintaining NGF bioactivity. Following intravenous administration, the radiolabeled NGF targets non-neuronal tissues (e.g., kidney, liver), and although absolute brain uptake was very low, the brain uptake that was observed was restricted to the cortex.

Indexed as

Nerve Growth FactorPositron-Emission TomographyPositron Emission Tomography Computed TomographyRadioisotopesAnimalsAutoradiographyBrainGallium RadioisotopesMaleMiceTissue DistributionZirconiumGallium-68Gallium RadioisotopesNerve Growth FactorRadioisotopesZirconiumZirconium-89BioconjugationBiodistributionChelatorNerve growth factorNGFPET imagingRadiolabelingRadiometalZirconium-89

Identifiers

PMID36692661
OpenAlexW4317871259

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.