Evidence map›Paper›PMID 36692641›Full record

ArticleClinical & translational oncology : official publication of the Federation of Spanish Oncology Societies and of the National Cancer Institute of Mexico2023

Bioinformatic analysis of single-cell RNA sequencing dataset dissects cellular heterogeneity of triple-negative breast cancer in transcriptional profile, splicing event and crosstalk network.

Jin Qiu, Lu Ma, Tingting Wang, Juntong Chen, Dongmei Wang, Yuhan Guo, Yin Li, Xinran Ma, Geng Chen, Ying Luo and 2 more

Abstract read
PubMed Publisher
In one paragraph

Article in Clinical & translational oncology : official publication of the Federation of Spanish Oncology Societies and of the National Cancer Institute of Mexico, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed, 1 pooled it
0.8field-weighted citation impact, top 28% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed, 1 synthesis or guideline pooled it, 5 citations in OpenAlex.

  1. Pooled it
  2. Review
  3. Review
  4. Article
  5. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors at 4 institutions in 1 country.

Jin Qiu *Department of Oncology, Shanghai Chest Hospital, Shanghai Jiaotong University, Shanghai, 200030, China.
Lu Ma *Shanghai Key Laboratory of Regulatory Biology, Institute of Biomedical Sciences and School of Life Sciences, East China Normal University, Shanghai, 200241, China.
Tingting Wang *Department of Anaesthesia, Changning Maternity and Infant Health Hospital, East China Normal University, Shanghai, 200050, China.
Juntong ChenShanghai Key Laboratory of Regulatory Biology, Institute of Biomedical Sciences and School of Life Sciences, East China Normal University, Shanghai, 200241, China.
Dongmei WangShanghai Key Laboratory of Regulatory Biology, Institute of Biomedical Sciences and School of Life Sciences, East China Normal University, Shanghai, 200241, China.
Yuhan GuoShanghai Key Laboratory of Regulatory Biology, Institute of Biomedical Sciences and School of Life Sciences, East China Normal University, Shanghai, 200241, China.
Yin LiDepartment of Oncology, Shanghai Chest Hospital, Shanghai Jiaotong University, Shanghai, 200030, China.
Xinran MaShanghai Key Laboratory of Regulatory Biology, Institute of Biomedical Sciences and School of Life Sciences, East China Normal University, Shanghai, 200241, China.
Geng ChenShanghai Key Laboratory of Regulatory Biology, Institute of Biomedical Sciences and School of Life Sciences, East China Normal University, Shanghai, 200241, China.
Ying LuoPrenatal Diagnosis Center, Department of Clinical Laboratory, Changning Maternity and Infant Health Hospital, East China Normal University, Shanghai, 200050, China. louise_4ever@163.com.
Xinghua ChengDepartment of Oncology, Shanghai Chest Hospital, Shanghai Jiaotong University, Shanghai, 200030, China. chengxinghua_001@163.com.
Lingyan XuShanghai Key Laboratory of Regulatory Biology, Institute of Biomedical Sciences and School of Life Sciences, East China Normal University, Shanghai, 200241, China. lyxu@bio.ecnu.edu.cn.ORCID http://orcid.org/0000-0002-8666-5219
East China Normal University · CNShanghai Changning Maternity and Infant Health Hospital · CNShanghai Chest Hospital · CNChongqing Normal University · CN

Funding

Clinical Research Plan of SHDC SHDC2022CRD025National Key Research and Development Program of China 2019YFA09004500National Natural Science Foundation of China 32022034National Natural Science Foundation of China 32071148National Natural Science Foundation of China 32222024National Natural Science Foundation of China 32271224National Natural Science Foundation of China 81502480National Natural Science Foundation of China 81972709National Natural Science Foundation of China 82073191Natural Science Foundation of Chongqing 33906003Natural Science Foundation of Shanghai 21140904300Natural Science Foundation of Shanghai 22ZR1421200Shanghai Chest Hospital Project of Collaborative Innovation YJXT20190105
6 · The paper itself

Abstract

backgroundTriple-negative breast cancer (TNBC) is a subtype of breast cancer with high tumoral heterogeneity, while the detailed regulatory network is not well known.

methodsVia single-cell RNA-sequencing (scRNA-seq) data analysis, we comprehensively investigated the transcriptional profile of different subtypes of TNBC epithelial cells with gene regulatory network (GRN) and alternative splicing (AS) event analysis, as well as the crosstalk between epithelial and non-epithelial cells.

resultsOf note, we found that luminal progenitor subtype exhibited the most complex GRN and splicing events. Besides, hnRNPs negatively regulates AS events in luminal progenitor subtype. In addition, we explored the cellular crosstalk among endothelial cells, stromal cells and immune cells in TNBC and discovered that NOTCH4 was a key receptor and prognostic marker in endothelial cells, which provide potential biomarker and target for TNBC intervention.

conclusionsIn summary, our study elaborates on the cellular heterogeneity of TNBC, revealing that NOTCH4 in endothelial cells was critical for TNBC intervention. This in-depth understanding of epithelial cell and non-epithelial cell network would provide theoretical basis for the development of new drugs targeting this sophisticated network in TNBC.

Indexed as

Triple Negative Breast NeoplasmsAlternative SplicingComputational BiologyEndothelial CellsHumansSequence Analysis, RNAEpithelial cellNetworkNon-epithelial cellscRNA-seqTNBCTumor heterogeneity

Identifiers

PMID36692641
OpenAlexW4317831339

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.