Evidence map›Paper›PMID 36689037›Full record

ArticleLasers in medical science2023

In vitro antibacterial activity of green tea-loaded chitosan nanoparticles on caries-related microorganisms and dentin after Er:YAG laser caries removal.

Fabiana Almeida Curylofo-Zotti, Viviane De Cássia Oliveira, Analu Rodriguez Marchesin, Hiago Salge Borges, Antonio Claudio Tedesco, Silmara Aparecida Milori Corona

Open access · greenAbstract read
PubMed Publisher
In one paragraph

Article in Lasers in medical science, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
1.4field-weighted citation impact, top 20% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed, 6 citations in OpenAlex.

  1. Trial
  2. Review
  3. Review
  4. Review
  5. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 2 institutions in 1 country.

Fabiana Almeida Curylofo-ZottiDepartment of Restorative Dentistry, School of Dentistry of Ribeirao Preto, University of Sao Paulo (USP), Cafe Avenue, s/n 14040-904, Sao Paulo, Ribeirao Preto, Brazil. fabianacurylofo@gmail.com.ORCID http://orcid.org/0000-0002-0482-6956
Viviane De Cássia OliveiraDepartment of Dental Materials and Prosthodontics, School of Dentistry of Ribeirao Preto, University of Sao Paulo (USP), Ribeirao Preto, Sao Paulo, SP, Brazil.
Analu Rodriguez MarchesinDepartment of Restorative Dentistry, School of Dentistry of Ribeirao Preto, University of Sao Paulo (USP), Cafe Avenue, s/n 14040-904, Sao Paulo, Ribeirao Preto, Brazil.
Hiago Salge BorgesDepartment of Chemistry, Center of Nanotechnology and Tissue Engineering - Photobiology and Photomedicine Research Group, Faculty of Philosophy, Sciences and Letters of Ribeirao Preto, University of Sao Paulo (USP), São Paulo, Brazil.
Antonio Claudio TedescoDepartment of Chemistry, Center of Nanotechnology and Tissue Engineering - Photobiology and Photomedicine Research Group, Faculty of Philosophy, Sciences and Letters of Ribeirao Preto, University of Sao Paulo (USP), São Paulo, Brazil.
Silmara Aparecida Milori CoronaDepartment of Restorative Dentistry, School of Dentistry of Ribeirao Preto, University of Sao Paulo (USP), Cafe Avenue, s/n 14040-904, Sao Paulo, Ribeirao Preto, Brazil.
Universidade de São Paulo · BRUniversidade de Ribeirão Preto · BR

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

This study aimed to determine the inhibitory effects of green tea (Gt), EGCG, and nanoformulations containing chitosan (Nchi) and chitosan+green tea (Nchi+Gt) against Streptococcus mutans and Lactobacillus casei. In addition, the antibacterial effect of nanoformulations was evaluated directly on dentin after the selective removal of carious lesion. At first, the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) against S. mutans and L. casei isolates were investigated. In parallel, dentin specimens were exposed to S. mutans to induce carious lesions. Soft dentin was selectively removed by Er:YAG laser (n=33) or bur (n=33). Remaining dentin was biomodified with Nchi (n=11) or Gt+Nchi (n=11). Control group (n=11) did not receive any treatment. Dentin scraps were collected at three time points. Microbiological analyses were conducted and evaluated by agar plate counts. Gt at 1:32 dilution inhibited S. mutans growth while 1:16 was efficient against L. casei. EGCG at 1:4 dilution completely inhibited S. mutans and L. casei growth. Independently of the association with Gt, Nchi completely inhibited S. mutans at 1:4 dilution. For L. casei, different concentrations of Nchi (1:32) and Nchi+Gt (1:8) were required to inhibit cell growth. After selective carious removal, viability of S. mutans decreased (p<0.001), without difference between bur and Er:YAG laser (p>0.05). Treatment with Nchi and Nchi+Gt did not influence the microbial load of S. mutans on dentin (p>0.05). Although variations in concentrations were noticed, all compounds showed antibacterial activity against S. mutans and L. casei. Both bur and Er:YAG laser have effectively removed soft dentin and reduced S. mutans counts. Nanoformulations did not promote any additional antibacterial effect in the remaining dentin.

Indexed as

ChitosanDental CariesLasers, Solid-StateAnti-Bacterial AgentsDental Caries SusceptibilityDentinHumansStreptococcus mutansAnti-Bacterial AgentsChitosanBurLactobacillus caseiNanoformulation, Er:YAG laser, High-power lasersStreptococcus mutans

Identifiers

PMID36689037
OpenAlexW4317781135

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.