ArticleFrontiers in microbiology2022
Automated flow cytometry as a tool to obtain a fine-grain picture of marine prokaryote community structure along an entire oceanographic cruise.
Article in Frontiers in microbiology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
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5 citing papers in PubMed.
- Spatial Partitioning of Microbial Functional Potential Suggests Complex Nitrogen Cycling in a Subterranean Estuary.Environmental microbiology · 2026Article
- A double-staining automated flow cytometry method for real-time monitoring of bacteria in continuous bioreactors.NPJ systems biology and applications · 2026Article
- Portable Lab for Shipping (POLS): A Biosensor-Based System for Rapid Onboard Detection ofMicroorganisms · 2025Article
- Impact of Submarine Groundwater Discharge on Microbial Communities in a Highly Anthropized Coastal Lagoon (Mar Menor, SE Spain).Molecular ecology · 2025Article
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Abstract
On a standard oceanographic cruise, flow cytometry data are usually collected sparsely through a bottle-based sampling and with stations separated by kilometers leading to a fragmented view of the ecosystem; to improve the resolution of the datasets produced by this technique here it is proposed the application of an automatic method of sampling and staining. The system used consists of a flow-cytometer (Accuri-C6) connected to an automated continuous sampler (OC-300) that collects samples of marine surface waters every 15 min. We tested this system for five days during a brief Mediterranean cruise with the aim of estimating the abundance, relative size and phenotypic diversity of prokaryotes. Seawater was taken by a faucet linked to an inlet pump (
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