Evidence map›Paper›PMID 36687598›Full record

ArticleFrontiers in microbiology2022

Tracking defined microbial communities by multicolor flow cytometry reveals tradeoffs between productivity and diversity.

Firas S Midani, Lawrence A David

Abstract read
In one paragraph

Article in Frontiers in microbiology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed.

  1. Article
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  4. Review
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors.

Firas S MidaniCenter for Genomic and Computational Biology, Duke University, Durham, NC, United States.
Lawrence A DavidCenter for Genomic and Computational Biology, Duke University, Durham, NC, United States.

Funding

Research Training in Pediatrics GastroenterolgyT32DK007664 · NIDDK · BAYLOR COLLEGE OF MEDICINE · PI DOUGLAS G BURRIN · 1991 to 2026
$8.4M
Personalizing prebiotic therapies that target human gut microbiotaR01DK116187 · NIDDK · DUKE UNIVERSITY · PI Lawrence Anthony David · 2018 to 2026
$3.7M
Using DNA sequencing to assess dietary species richnessR01DK128611 · NIDDK · DUKE UNIVERSITY · PI Lawrence Anthony David · 2022 to 2026
$2.9M
NIDDK NIH HHS R01 DK116187NIDDK NIH HHS R01 DK128611NIDDK NIH HHS T32 DK007664
6 · The paper itself

Abstract

Cross feeding between microbes is ubiquitous, but its impact on the diversity and productivity of microbial communities is incompletely understood. A reductionist approach using simple microbial communities has the potential to detect cross feeding interactions and their impact on ecosystem properties. However, quantifying abundance of more than two microbes in a community in a high throughput fashion requires rapid, inexpensive assays. Here, we show that multicolor flow cytometry combined with a machine learning-based classifier can rapidly quantify species abundances in simple, synthetic microbial communities. Our approach measures community structure over time and detects the exchange of metabolites in a four-member community of fluorescent

Indexed as

Bacteroidescarbon sourcescross feedingflow cytometrymicrobial ecology

Identifiers

PMID36687598
PMCPMC9849913

What OpenQuestion holds

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LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.