ArticleFrontiers in endocrinology2022
A new perspective on semen quality of aged male: The characteristics of metabolomics and proteomics.
Article in Frontiers in endocrinology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers, 1 of them a synthesis that pooled it.
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Who cites it
22 citing papers in PubMed, 1 synthesis or guideline pooled it, 35 citations in OpenAlex.
- Male Infertility and Neurodegenerative Diseases: A Systematic Review of Associations and Molecular Mechanisms.International journal of molecular sciences · 2026Pooled it
- Age-Associated Metabolomic Changes in Human Spermatozoa.International journal of molecular sciences · 2026Article
- Integrated metabolomic and microbiota analysis of semen: seasonal and morphological associations.Asian journal of andrology · 2026Article
- Exploratory seminal plasma metabolomics and independent HPLC-UV evaluation in men with impaired semen quality.Frontiers in endocrinology · 2026Article
- Review
- Metabolomic profiling and machine learning-based biomarker identification for oligoasthenozoospermia.Metabolomics : Official journal of the Metabolomic Society · 2025Article
- Untargeted metabolomics reveals changes in boar sperm and seminal plasma metabolites associated with sexual maturity.Journal of animal science and biotechnology · 2025Article
- Intra-individual variability of the human seminal plasma metabolome.Asian journal of andrology · 2025Article
- Integrated seminal plasma metabolomics and lipidomics profiling highlight distinctive signature of varicocele patients with male infertility.Asian journal of andrology · 2025Article
- Integrative pigGTEx resource with GWAS reveals genetic mechanism underlying semen quality in boars.Journal of animal science and biotechnology · 2025Article
- Sperm metabolomic signatures of asthenozoospermia and teratozoospermia in Chinese reproductive-age men.Scientific reports · 2025Article
- Age-Associated Proteomic Changes in Human Spermatozoa.International journal of molecular sciences · 2025Article
- Article
- Proteomic analysis reveals the difference between the spermatozoa of young and old Sus scrofa.Scientific reports · 2025Article
- Proteostasis as a Sentry for Sperm Quality and Male Fertility.Advances in experimental medicine and biology · 2025Review
- Effect and mechanism of modified Yougui power on Simmental bulls with oligoasthenozoospermia based on targeted amino acid metabolism.Frontiers in veterinary science · 2025Article
- Extracellular Vesicles in the Aging Male Reproductive System: Progresses and Perspectives.Advances in experimental medicine and biology · 2025Review
- Ambient Ozone Exposure, Semen Plasma Metabolites, and Sperm Quality Decline among Adult Men in Wuhan China.Environment & health (Washington, D.C.) · 2024Article
- Advanced Paternal Age in Focus: Unraveling Its Influence on Assisted Reproductive Technology Outcomes.Journal of clinical medicine · 2024Review
- Predicting Male Infertility Using Artificial Neural Networks: A Review of the Literature.Healthcare (Basel, Switzerland) · 2024Review
Corrections and comments
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Authors and funding
10 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Background: Semen quality is negatively correlated with male age and is mainly quantified by a routine semen analysis, which is descriptive and inconclusive. Sperm proteins or semen metabolites are used as the intermediate or end-products, reflecting changes in semen quality, and hold much promise as a new biomarker to predict fertility in advanced-aged males. Objectives: In this study, we sought to assess whether the semen metabolome and proteome of aged males can affect semen quality and serve as biomarkers for predicting semen quality. Materials and methods: We retrospectively analyzed 12825 males that underwent semen routine analysis to understand the age-dependent changes in sperm quality. To identify the difference between aged and young adults, metabolomics (n=60) analyses of semen and proteomics (n=12) analyses of sperm were conducted. Finally, integrated machine learning of metabolomics was conducted to screen biomarkers to identify aging semen. Results: We discovered that male age was positively correlated with sperm concentration as well as DNA fragmentation index(DFI), and negatively with progressive motile sperm count, total sperm count, sperm volume and progressive sperm motility. The differential metabolites were significantly enriched in various metabolic pathways, and four of these differential metabolites (Pipamperone, 2,2-Bis(hydroxymethyl)-2,2',2''-nitrilotriethanol, Arg-Pro and Triethyl phosphate) were utilized to establish a biomarker panel to identify aging semen. Proteomic analysis showed that differential proteins were significantly enriched in protein digestion and absorption and some energy-related pathways. An integrated analysis of the metabolome and proteome identified differential energy metabolism and oxidative stress-related proteins, which could explain the decreased motility and the increased DFI of aging sperm. Discussion and conclusion: We provide compelling evidence that the changes in semen metabolome and sperm proteome are related to the decline of semen quality in aged males. Moreover, a biomarker panel based on four metabolites was established to identify aging semen.
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