Evidence map›Paper›PMID 36682882›Full record

ArticleMicrobiology spectrum2023

Rapid Diagnostic Tests for the Detection of the Four Dengue Virus Serotypes in Clinically Relevant Matrices.

Nina M Pollak, Malin Olsson, Madeeha Ahmed, Javier Tan, George Lim, Yin Xiang Setoh, Judith Chui Ching Wong, Yee Ling Lai, Jody Hobson-Peters, Joanne Macdonald and 1 more

Open access · goldAbstract read
In one paragraph

Article in Microbiology spectrum, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.

0numbers the graph read from it
0cells of the map it votes in
18citing papers in PubMed
8.7field-weighted citation impact, top 2% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

18 citing papers in PubMed, 30 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors at 4 institutions in 2 countries.

Nina M PollakCentre for Bioinnovation, University of the Sunshine Coast, Sippy Downs, Queensland, Australia.ORCID 0000-0001-8437-3303
Malin OlssonCentre for Bioinnovation, University of the Sunshine Coast, Sippy Downs, Queensland, Australia.
Madeeha AhmedCentre for Bioinnovation, University of the Sunshine Coast, Sippy Downs, Queensland, Australia.
Javier TanEnvironmental Health Institute, National Environment Agency, Singapore, Singapore.
George LimEnvironmental Health Institute, National Environment Agency, Singapore, Singapore.
Yin Xiang SetohEnvironmental Health Institute, National Environment Agency, Singapore, Singapore.
Judith Chui Ching WongEnvironmental Health Institute, National Environment Agency, Singapore, Singapore.
Yee Ling LaiEnvironmental Health Institute, National Environment Agency, Singapore, Singapore.
Jody Hobson-PetersSchool of Chemistry and Molecular Biosciences, The University of Queensland, St. Lucia, Queensland, Australia.
Joanne MacdonaldDMTC Ltd., Kew, Victoria, Australia.
David McMillanCentre for Bioinnovation, University of the Sunshine Coast, Sippy Downs, Queensland, Australia.
National Environment Agency · SGUniversity of the Sunshine Coast · AUDefence Materials Technology Centre (Australia) · AUThe University of Queensland · AU

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The efficient and accurate diagnosis of dengue, a major mosquito-borne disease, is of primary importance for clinical care, surveillance, and outbreak control. The identification of specific dengue virus serotype 1 (DENV-1) to DENV-4 can help in understanding the transmission dynamics and spread of dengue disease. The four rapid low-resource serotype-specific dengue tests use a simple sample preparation reagent followed by reverse transcription-isothermal recombinase polymerase amplification (RT-RPA) combined with lateral flow detection (LFD) technology. Results are obtained directly from clinical sample matrices in 35 min, requiring only a heating block and pipettes for liquid handling. In addition, we demonstrate that the rapid sample preparation step inactivates DENV, improving laboratory safety. Human plasma and serum were spiked with DENV, and DENV was detected with analytical sensitivities of 333 to 22,500 median tissue culture infectious doses (TCID

Indexed as

DengueDengue VirusHumansRapid Diagnostic TestsRecombinasesSensitivity and SpecificitySerogroupRecombinasesdengue virusisothermal amplificationlateral flow detectionNS5 generapid molecular assaysrapid sample preparationrecombinase polymerase amplification

Identifiers

PMID36682882
PMCPMC9927141
OpenAlexW4317716393

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.