Evidence map›Paper›PMID 36682867›Full record

ArticleMethods in enzymology2023

Generation of nucleotide-linked resins for identification of novel binding proteins.

Shikha S Chauhan, Emily E Weinert

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Methods in enzymology, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors.

Shikha S ChauhanDepartment of Biochemistry and Molecular Biology, Pennsylvania State University, University Park, PA, United States.
Emily E WeinertDepartment of Biochemistry and Molecular Biology, Pennsylvania State University, University Park, PA, United States; Department of Chemistry, Pennsylvania State University, University Park, PA, United States. Electronic address: emily.weinert@psu.edu.

Funding

Probing the Metabolism and Cellular Roles of 2',3'-Cyclic Nucleotide MonophosphateR01GM125842 · NIGMS · PENNSYLVANIA STATE UNIVERSITY, THE · PI WEINERT, EMILY E · 2018 to 2022
$1.6M
NIGMS NIH HHS R01 GM125842
6 · The paper itself

Abstract

Organisms use numerous nucleotide-containing compounds as intracellular signals to control behavior. Identifying the biomolecules responsible to sensing and responding to changes in signaling molecule concentration is an important area of research. However, identifying the binding proteins can be challenging when there is no prior information available about binding motifs. In this chapter, we describe a straightforward method to generate nucleotide-linked resins for use in pull-down experiments to identify binding proteins. The protocol outlined in this chapter also can be adapted to generate custom resins linked to other molecules of interest.

Indexed as

Carrier ProteinsNucleotidesSignal TransductionCarrier ProteinsNucleotides2′,3′-cyclic nucleotide monophosphateBinding proteinsCyclic nucleotideNucleotide couplingNucleotide resinNucleotide signalingProtein pull-downPull-down

Identifiers

PMID36682867
PMCPMC13556980

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.