Evidence map›Paper›PMID 36680182›Full record

ArticleViruses2023

Differential Susceptibility of Fetal Retinal Pigment Epithelial Cells, hiPSC- Retinal Stem Cells, and Retinal Organoids to Zika Virus Infection.

Deisy Contreras, Gustavo Garcia, Melissa Kaye Jones, Laura E Martinez, Akshaya Jayakarunakaran, Vineela Gangalapudi, Jie Tang, Ying Wu, Jiagang J Zhao, Zhaohui Chen and 6 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Viruses, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
2.6field-weighted citation impact, top 11% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 9 citations in OpenAlex.

  1. Article
  2. NovelInternational journal of ophthalmology · 2025
    Article
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

16 authors at 4 institutions in 2 countries.

Deisy ContrerasBoard of Governors Regenerative Medicine Institute, Cedars-Sinai Medical Center, Los Angeles, CA 90048, USA.
Gustavo GarciaDepartment of Molecular and Medical Pharmacology, University of California, Los Angeles, CA 90095, USA.ORCID 0000-0002-1649-9251
Melissa Kaye JonesBoard of Governors Regenerative Medicine Institute, Cedars-Sinai Medical Center, Los Angeles, CA 90048, USA.
Laura E MartinezDepartment of Molecular and Medical Pharmacology, University of California, Los Angeles, CA 90095, USA.
Akshaya JayakarunakaranDepartment of Molecular and Medical Pharmacology, University of California, Los Angeles, CA 90095, USA.
Vineela GangalapudiGenomics Core, Cedars-Sinai Medical Center, Los Angeles, CA 90048, USA.
Jie TangDepartment of Molecular and Medical Pharmacology, University of California, Los Angeles, CA 90095, USA.
Ying WuAlpine BioTherapeutics Corporation, 11107 Roselle Street, Suite 210, San Diego, CA 92121, USA.
Jiagang J ZhaoAlpine BioTherapeutics Corporation, 11107 Roselle Street, Suite 210, San Diego, CA 92121, USA.
Zhaohui ChenDepartment of Biomedical Sciences, Cedars-Sinai Medical Center, Los Angeles, CA 90048, USA.
Arunachalam RamaiahTata Institute for Genetics and Society, Center at inStem, Bangalore 560065, India.ORCID 0000-0003-3573-6141
Irena TsuiRetina Division, Department of Ophthalmology, University of California at Los Angeles, Los Angeles, CA 90095, USA.
Ashok KumarDepartment of Ophthalmology, Visual and Anatomical Sciences, Wayne State University, Detroit, MI 48201, USA.ORCID 0000-0001-6332-6591
Karin Nielsen-SainesDepartment of Pediatrics, University of California, Los Angeles, CA 90095, USA.ORCID 0000-0002-1742-5211
Shaomei WangBoard of Governors Regenerative Medicine Institute, Cedars-Sinai Medical Center, Los Angeles, CA 90048, USA.ORCID 0000-0002-4605-8166
Vaithilingaraja ArumugaswamiBoard of Governors Regenerative Medicine Institute, Cedars-Sinai Medical Center, Los Angeles, CA 90048, USA.
Cedars-Sinai Medical Center · USUniversity of California, Los Angeles · USInstitute for Stem Cell Biology and Regenerative Medicine · INWayne State University · US

Funding

Metabolic and epigenetic reprogramming of vital organs in SARS-CoV-2 induced systemic toxicityR01DK132735 · NIDDK · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI ARUMUGASWAMI, VAITHILINGARAJA, DEB, ARJUN · 2021 to 2023
$6.0M
Genetic evolution, pathogenesis and immune responses in mother to child transmission of ZIKVR01AI140718 · NIAID · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI CHENG, GENHONG, JUNG, JAE U · 2018 to 2022
$3.8M
Interplay between AMPK and Hippo Signaling Regulates Ocular Antiviral Response to Zika virus infectionR01EY032149 · NEI · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI Vaithilingaraja Arumugaswami, Ashok Kumar · 2021 to 2026
$2.8M
Developing three-dimensional antisense oligonucleotide drugs against COVID-19R01AI163216 · NIAID · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI GUO, FENG · 2021 to 2025
$2.3M
ADAR1-mediated antiviral response in Zika virus (ZIKV) infectionR21AI149385 · NIAID · WAYNE STATE UNIVERSITY · PI KUMAR, ASHOK · 2022 to 2023
$424k
Opthalmic Manifestations of Congenital Zika Virus InfectionR21EY028318 · NEI · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI BRASIL, PATRICIA, NIELSEN, KARIN · 2017 to 2018
$373k
NEI NIH HHS R01 EY032149NEI NIH HHS R21 EY028318NIAID NIH HHS R01 AI140718NIAID NIH HHS R01 AI163216NIAID NIH HHS R21 AI149385NIDDK NIH HHS R01 DK132735
6 · The paper itself

Abstract

Zika virus (ZIKV) causes microcephaly and congenital eye disease. The cellular and molecular basis of congenital ZIKV infection are not well understood. Here, we utilized a biologically relevant cell-based system of human fetal retinal pigment epithelial cells (FRPEs), hiPSC-derived retinal stem cells (iRSCs), and retinal organoids to investigate ZIKV-mediated ocular cell injury processes. Our data show that FRPEs were highly susceptible to ZIKV infection exhibiting increased apoptosis, whereas iRSCs showed reduced susceptibility. Detailed transcriptomics and proteomics analyses of infected FRPEs were performed. Nucleoside analogue drug treatment inhibited ZIKV replication. Retinal organoids were susceptible to ZIKV infection. The Asian genotype ZIKV exhibited higher infectivity, induced profound inflammatory response, and dysregulated transcription factors involved in retinal organoid differentiation. Collectively, our study shows that ZIKV affects ocular cells at different developmental stages resulting in cellular injury and death, further providing molecular insight into the pathogenesis of congenital eye disease.

Indexed as

Eye DiseasesInduced Pluripotent Stem CellsZika VirusZika Virus InfectionEpithelial CellsHumansOrganoidsRetinaRetinal PigmentsVirus ReplicationRetinal Pigmentsapoptosiscongenital eye diseasehuman fetal retinal pigment epithelial cellshuman iPSC-derived retinal stem cellsnucleoside analogueretinal organoidsZika virus

Identifiers

PMID36680182
PMCPMC9864143
OpenAlexW4313472835

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.