Evidence map›Paper›PMID 36678495›Full record

ArticlePathogens (Basel, Switzerland)2023

Identification of

Ayanda Manqele, Nomakorinte Gcebe, Rian Ewald Pierneef, Rebone Moerane, Abiodun Adewale Adesiyun

Open access · goldAbstract read
In one paragraph

Article in Pathogens (Basel, Switzerland), 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
1.9field-weighted citation impact, top 16% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 7 citations in OpenAlex.

  1. Article
  2. Article
  3. Genomic Characterization ofPathogens (Basel, Switzerland) · 2023
    Article
  4. A review of the literature ofFrontiers in microbiology · 2023
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 1 institution in 2 countries.

Ayanda ManqeleDepartment of Production Animal Studies, Faculty of Veterinary Science, University of Pretoria, Onderstepoort, Pretoria 0110, South Africa.
Nomakorinte GcebeDepartment of Production Animal Studies, Faculty of Veterinary Science, University of Pretoria, Onderstepoort, Pretoria 0110, South Africa.
Rian Ewald PierneefAgricultural Research Council-Biotechnology Platform, Private Bag X05, Ondersterpoort, Pretoria 0110, South Africa.ORCID 0000-0002-0619-0058
Rebone MoeraneDepartment of Production Animal Studies, Faculty of Veterinary Science, University of Pretoria, Onderstepoort, Pretoria 0110, South Africa.
Abiodun Adewale AdesiyunDepartment of Production Animal Studies, Faculty of Veterinary Science, University of Pretoria, Onderstepoort, Pretoria 0110, South Africa.ORCID 0000-0001-9470-9421
Onderstepoort Veterinary Academic Hospital · ZA

Funding

Red Meat Research and Development South Africa (RMRDSA) N/A
6 · The paper itself

Abstract

In this study, Listeria isolates (214) were characterized as follows: L. innocua (77.10%), L. monocytogenes (11.21%), L. welshimeri (5.61%), L. grayi (1.40%), L. seeligeri (0.93%), and L. species (3.73%) that were not identified at the species level, from beef and beef based products from retail and farms in Mpumalanga and North West provinces of South Africa. MLVA was further used to type Listeria innocua isolates (165) and Listeria monocytogenes isolates (24). The L. monocytogenes isolates were also serogrouped using PCR. The MLVA protocol for L. monocytogenes typing included six tandem repeat primer sets, and the MLVA protocol for L. innocua included the use of three tandem repeats primer sets. The L. monocytogenes serogroups were determined as follows: 4b-4d-4e (IVb) (37.50%), 1/2a-3a (IIa) (29.16%), 1/2b-3b (IIb) (12.50%), 1/2c-3c (IIc) (8.33%), and IVb-1 (4.16%). MLVA could cluster isolates belonging to each specie, L. monocytogenes, and L. innocua isolates, into MLVA-related strains. There were 34 and 10 MLVA types obtained from the MLVA typing of L. innocua and L. monocytogenes, respectively. MLVA clustered the L. monocytogenes isolates irrespective of sample category, serogroups, and geographical origin. Similarly, the L. innocua isolates clustered irrespective of meat category and geographical origin. MLVA was able to cluster isolates based on MLVA relatedness. The clustering of isolates from farms and retailers indicates transmission of Listeria spp. MLVA is an affordable, simple, and discriminatory method that can be used routinely to type L. monocytogenes and L. innocua isolates.

Indexed as

farmsL. innocuaListeria speciesL. monocytogenesMLVAretailersserogrouping

Identifiers

PMID36678495
PMCPMC9862459
OpenAlexW4316372834

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.