Evidence map›Paper›PMID 36677477›Full record

ArticleMicroorganisms2023

Examining Different Analysis Protocols Targeting Hospital Sanitary Facility Microbiomes.

Claudio Neidhöfer, Esther Sib, Al-Harith Benhsain, Christina Mutschnik-Raab, Anna Schwabe, Alexander Wollkopf, Nina Wetzig, Martin A Sieber, Ralf Thiele, Manuel Döhla and 3 more

Abstract read
In one paragraph

Article in Microorganisms, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.

0numbers the graph read from it
0cells of the map it votes in
7citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

7 citing papers in PubMed.

  1. Article
  2. Article
  3. Article
  4. Article
  5. ESKAPEE Pathogen Biofilm Control on Surfaces with ProbioticAntibiotics (Basel, Switzerland) · 2023
    Review
  6. Article
  7. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors.

Claudio NeidhöferInstitute of Medical Microbiology, Immunology and Parasitology, University Hospital Bonn, Venusberg-Campus 1, 53127 Bonn, Germany.ORCID 0000-0002-2064-3885
Esther SibInstitute for Hygiene and Public Health, University of Bonn, 53127 Bonn, Germany.
Al-Harith BenhsainInstitute of Medical Microbiology, Immunology and Parasitology, University Hospital Bonn, Venusberg-Campus 1, 53127 Bonn, Germany.
Christina Mutschnik-RaabHospital Pharmacy, Bundeswehr Central Hospital Koblenz, 56072 Koblenz, Germany.
Anna SchwabeInstitute for Hygiene and Public Health, University of Bonn, 53127 Bonn, Germany.ORCID 0000-0002-7463-5135
Alexander WollkopfInstitute for Hygiene and Public Health, University of Bonn, 53127 Bonn, Germany.ORCID 0000-0003-1589-6412
Nina WetzigInstitute for Functional Gene Analytics, Bonn-Rhein-Sieg University of Applied Sciences, 53757 Sankt Augustin, Germany.
Martin A SieberInstitute for Functional Gene Analytics, Bonn-Rhein-Sieg University of Applied Sciences, 53757 Sankt Augustin, Germany.
Ralf ThieleInstitute for Functional Gene Analytics, Bonn-Rhein-Sieg University of Applied Sciences, 53757 Sankt Augustin, Germany.
Manuel DöhlaInstitute for Hygiene and Public Health, University of Bonn, 53127 Bonn, Germany.ORCID 0000-0001-8029-5264
Steffen EngelhartInstitute for Hygiene and Public Health, University of Bonn, 53127 Bonn, Germany.
Nico T MuttersInstitute for Hygiene and Public Health, University of Bonn, 53127 Bonn, Germany.
Marijo ParčinaInstitute of Medical Microbiology, Immunology and Parasitology, University Hospital Bonn, Venusberg-Campus 1, 53127 Bonn, Germany.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Indoor spaces exhibit microbial compositions that are distinctly dissimilar from one another and from outdoor spaces. Unique in this regard, and a topic that has only recently come into focus, is the microbiome of hospitals. While the benefits of knowing exactly which microorganisms propagate how and where in hospitals are undoubtedly beneficial for preventing hospital-acquired infections, there are, to date, no standardized procedures on how to best study the hospital microbiome. Our study aimed to investigate the microbiome of hospital sanitary facilities, outlining the extent to which hospital microbiome analyses differ according to sample-preparation protocol. For this purpose, fifty samples were collected from two separate hospitals-from three wards and one hospital laboratory-using two different storage media from which DNA was extracted using two different extraction kits and sequenced with two different primer pairs (V1-V2 and V3-V4). There were no observable differences between the sample-preservation media, small differences in detected taxa between the DNA extraction kits (mainly concerning

Indexed as

16S rRNA gene sequencingbuilt environmenthigh-throughput DNA sequencinghospital-acquired infectionshospital environmentmicrobial ecologymicrobiomeNGS

Identifiers

PMID36677477
PMCPMC9867353

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.