Evidence map›Paper›PMID 36677403›Full record

ArticleMicroorganisms2022

Hybrid Genomic Analysis of

Danitza Xiomara Romero-Calle, Francisnei Pedrosa-Silva, Luiz Marcelo Ribeiro Tomé, Thiago J Sousa, Leila Thaise Santana de Oliveira Santos, Vasco Ariston de Carvalho Azevedo, Bertram Brenig, Raquel Guimarães Benevides, Thiago M Venancio, Craig Billington and 1 more

Open access · goldAbstract read
In one paragraph

Article in Microorganisms, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
0.4field-weighted citation impact, top 33% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 3 citations in OpenAlex.

  1. Article
  2. Genetic Diversity ofPathogens (Basel, Switzerland) · 2024
    Article
  3. Molecular Characterization ofMicroorganisms · 2023
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors at 5 institutions in 3 countries.

Danitza Xiomara Romero-CallePostgraduate Program in Biotechnology, State University of Feira de Santana (UEFS), Av. Transnordestina S/N, Feira de Santana 44036-900, BA, Brazil.
Francisnei Pedrosa-SilvaLaboratory of Chemistry, Function of Proteins and Peptides, Center for Biosciences and Biotechnology, Darcy Ribeiro North Fluminense State University (UENF), Campos dos Goytacazes 28013-602, RJ, Brazil.ORCID 0000-0001-6394-7590
Luiz Marcelo Ribeiro ToméMolecular and Computational Biology of Fungi Laboratory, Department of Microbiology, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte 31270-901, MG, Brazil.
Thiago J SousaLaboratory of Cellular and Molecular Genetics, Department of Genetics, Ecology and Evolution, Institute of Biological Sciences, Federal University of Minas Gerais, Belo Horizonte 31270-901, MG, Brazil.
Leila Thaise Santana de Oliveira SantosDepartment of Biological Sciences, Feira de Santana State University (UEFS), Feira de Santana 44036-900, BA, Brazil.
Vasco Ariston de Carvalho AzevedoLaboratory of Cellular and Molecular Genetics, Department of Genetics, Ecology and Evolution, Institute of Biological Sciences, Federal University of Minas Gerais, Belo Horizonte 31270-901, MG, Brazil.ORCID 0000-0002-4775-2280
Bertram BrenigInstitute of Veterinary Medicine, Burckhardtweg, University of Göttingen, 37073 Göttingen, Germany.ORCID 0000-0002-7635-9656
Raquel Guimarães BenevidesPostgraduate Program in Biotechnology, State University of Feira de Santana (UEFS), Av. Transnordestina S/N, Feira de Santana 44036-900, BA, Brazil.
Thiago M VenancioLaboratory of Chemistry, Function of Proteins and Peptides, Center for Biosciences and Biotechnology, Darcy Ribeiro North Fluminense State University (UENF), Campos dos Goytacazes 28013-602, RJ, Brazil.ORCID 0000-0002-2215-8082
Craig BillingtonHealth & Environment Group, Institute of Environmental Sciences and Research, P.O. Box 29-181, Christchurch 8540, New Zealand.ORCID 0000-0003-3433-4220
Aristóteles Góes-NetoPostgraduate Program in Biotechnology, State University of Feira de Santana (UEFS), Av. Transnordestina S/N, Feira de Santana 44036-900, BA, Brazil.ORCID 0000-0002-7692-6243
Universidade Federal de Minas Gerais · BRState University of Norte Fluminense · BRUniversidade Estadual de Feira de Santana · BRNew Zealand Institute for Public Health and Forensic Science · NZUniversity of Göttingen · DE

Funding

Coordenação de Aperfeicoamento de Pessoal de Nível Superior 001
6 · The paper itself

Abstract

In Brazil, Salmonella enterica serovar Enteritidis is a significant health threat. Salmonella enterica serovar Enteritidis SE3 was isolated from soil at the Subaé River in Santo Amaro, Brazil, a region contaminated with heavy metals and organic waste. Illumina HiSeq and Oxford Nanopore Technologies MinION sequencing were used for de novo hybrid assembly of the Salmonella SE3 genome. This approach yielded 10 contigs with 99.98% identity with S. enterica serovar Enteritidis OLF-SE2-98984-6. Twelve Salmonella pathogenic islands, multiple virulence genes, multiple antimicrobial gene resistance genes, seven phage defense systems, seven prophages and a heavy metal resistance gene were encoded in the genome. Pangenome analysis of the S. enterica clade, including Salmonella SE3, revealed an open pangenome, with a core genome of 2137 genes. Our study showed the effectiveness of a hybrid sequence assembly approach for environmental Salmonella genome analysis using HiSeq and MinION data. This approach enabled the identification of key resistance and virulence genes, and these data are important to inform the control of Salmonella and heavy metal pollution in the Santo Amaro region of Brazil.

Indexed as

antimicrobial resistanceheavy metalhybrid sequence assemblySalmonellawhole genome sequencing

Identifiers

PMID36677403
PMCPMC9861973
OpenAlexW4313472378

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.