ArticleThe Journal of cell biology1987
Cytoskeletal reorganization of human platelets after stimulation revealed by the quick-freeze deep-etch technique.
Article in The Journal of cell biology, 1987. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed, 70 citations in OpenAlex.
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- A circle of life: platelet and megakaryocyte cytoskeleton dynamics in health and disease.Open biology · 2024Review
- Injury-induced actin cytoskeleton reorganization in podocytes revealed by super-resolution microscopy.JCI insight · 2017Article
- Interplay of Platelet Contractility and Elasticity of Fibrin/Erythrocytes in Blood Clot Retraction.Biophysical journal · 2017Article
- MAP1B regulates axonal development by modulating Rho-GTPase Rac1 activity.Molecular biology of the cell · 2010Article
- LIMK1 regulates Golgi dynamics, traffic of Golgi-derived vesicles, and process extension in primary cultured neurons.Molecular biology of the cell · 2004Article
- Actin dynamics in platelets.International review of cytology · 2002Review
- Evidence for the involvement of Tiam1 in axon formation.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2001Article
- Presenilin-1 mutations reduce cytoskeletal association, deregulate neurite growth, and potentiate neuronal dystrophy and tau phosphorylation.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2001Article
- Slow axonal transport of neurofilament protein in cultured neurons.The Journal of cell biology · 1999Article
- Evidence for the participation of the neuron-specific CDK5 activator P35 during laminin-enhanced axonal growth.The Journal of neuroscience : the official journal of the Society for Neuroscience · 1998Article
- Suppression of radixin and moesin alters growth cone morphology, motility, and process formation in primary cultured neurons.The Journal of cell biology · 1998Article
- Tau binds to the distal axon early in development of polarity in a microtubule- and microfilament-dependent manner.The Journal of neuroscience : the official journal of the Society for Neuroscience · 1996Article
- Interaction of tau with the neural plasma membrane mediated by tau's amino-terminal projection domain.The Journal of cell biology · 1995Article
- Interaction of dynamin with microtubules: its structure and GTPase activity investigated by using highly purified dynamin.Molecular biology of the cell · 1992Article
- Mechanisms of actin rearrangements mediating platelet activation.The Journal of cell biology · 1992Article
- Cytoskeletal filaments in embryonic chick myocardial cells as revealed by the quick-freeze deep-etch method combined with immunocytochemistry.Cell and tissue research · 1991Article
- Formation and contraction of a microfilamentous shell in saponin-permeabilized platelets.The Journal of cell biology · 1991Article
- The cytoskeleton of the resting human blood platelet: structure of the membrane skeleton and its attachment to actin filaments.The Journal of cell biology · 1991Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We studied the cytoskeletal reorganization of saponized human platelets after stimulation by using the quick-freeze deep-etch technique, and examined the localization of myosin in thrombin-treated platelets by immunocytochemistry at the electron microscopic level. In unstimulated saponized platelets we observed cross-bridges between: adjoining microtubules, adjoining actin filaments, microtubules and actin filaments, and actin filaments and plasma membranes. After activation with 1 U/ml thrombin for 3 min, massive arrays of actin filaments with mixed polarity were found in the cytoplasm. Two types of cross-bridges between actin filaments were observed: short cross-bridges (11 +/- 2 nm), just like those observed in the resting platelets, and longer ones (22 +/- 3 nm). Actin filaments were linked with the plasma membrane via fine short filaments and sometimes ended on the membrane. Actin filaments and microtubules frequently ran close to the membrane organelles. We also found that actin filaments were associated by end-on attachments with some organelles. Decoration with subfragment 1 of myosin revealed that all the actin filaments associated end-on with the membrane pointed away in their polarity. Immunocytochemical study revealed that myosin was present in the saponin-extracted cytoskeleton after activation and that myosin was localized on the filamentous network. The results suggest that myosin forms a gel with actin filaments in activated platelets. Close associations between actin filaments and organelles in activated platelets suggests that contraction of this actomyosin gel could bring about the observed centralization of organelles.
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