Evidence map›Paper›PMID 36675118›Full record

ArticleInternational journal of molecular sciences2023

Two-Step Identification of N-, S-, R- and T-Cytoplasm Types in Onion Breeding Lines Using High-Resolution Melting (HRM)-Based Markers.

Ludmila Khrustaleva, Mais Nzeha, Aleksey Ermolaev, Ekaterina Nikitina, Valery Romanov

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in International journal of molecular sciences, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Ludmila KhrustalevaCenter of Molecular Biotechnology, Russian State Agrarian University-Moscow Timiryazev Agricultural Academy, 49, Timiryazevskaya Str., 127550 Moscow, Russia.ORCID 0000-0002-0614-4641
Mais NzehaCenter of Molecular Biotechnology, Russian State Agrarian University-Moscow Timiryazev Agricultural Academy, 49, Timiryazevskaya Str., 127550 Moscow, Russia.ORCID 0000-0001-5567-7631
Aleksey ErmolaevCenter of Molecular Biotechnology, Russian State Agrarian University-Moscow Timiryazev Agricultural Academy, 49, Timiryazevskaya Str., 127550 Moscow, Russia.ORCID 0000-0001-9789-8694
Ekaterina NikitinaAll-Russian Research Institute of Agricultural Biotechnology, Timiryazevskaya 42 Str., 127550 Moscow, Russia.ORCID 0000-0001-5002-8229
Valery RomanovFederal Scientific Vegetable Center, Selectionaya St. 14, VNIISSOK, Odintsovo Region, 143072 Moscow, Russia.ORCID 0000-0002-3287-1914

Funding

Ministry of Science and Higher Education of the Russian Federation 075-152022-317
6 · The paper itself

Abstract

High-resolution melting (HRM) analysis is a powerful detection method for fast, high-throughput post-PCR analysis. A two-step HRM marker system was developed for identification of the N-, S-, R- and T-cytoplasms of onion. In the first step for the identification of N-, S- and R-cytoplasms, one forward primer was designed to the identical sequences of both

Indexed as

OnionsPlant BreedingCytoplasmGenes, PlantPolymerase Chain Reactioncytoplasmic male-sterilityhigh-resolution melting (HRM)mitochondrial genesmolecular markersonion (Allium cepa L.)

Identifiers

PMID36675118
PMCPMC9866120

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.