Evidence map›Paper›PMID 36672832›Full record

ArticleGenes2022

An RNA Interference (RNAi) Toolkit and Its Utility for Functional Genetic Analysis of

Lon-Fye Lye, Katherine L Owens, Soojin Jang, Joseph E Marcus, Erin A Brettmann, Stephen M Beverley

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Genes, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
2.0field-weighted citation impact, top 14% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed, 10 citations in OpenAlex.

  1. Review
  2. Article
  3. Recent advances in the genetic engineering of theIranian journal of basic medical sciences · 2025
    Review
  4. Non-coding RNAs as emerging players inFrontiers in cellular and infection microbiology · 2025
    Article
  5. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 1 institution in 1 country.

Lon-Fye LyeDepartment of Molecular Microbiology, Washington University School of Medicine, St. Louis, MO 63110, USA.
Katherine L OwensDepartment of Molecular Microbiology, Washington University School of Medicine, St. Louis, MO 63110, USA.
Soojin JangDepartment of Molecular Microbiology, Washington University School of Medicine, St. Louis, MO 63110, USA.
Joseph E MarcusDepartment of Molecular Microbiology, Washington University School of Medicine, St. Louis, MO 63110, USA.ORCID 0000-0002-1789-603X
Erin A BrettmannDepartment of Molecular Microbiology, Washington University School of Medicine, St. Louis, MO 63110, USA.
Stephen M BeverleyDepartment of Molecular Microbiology, Washington University School of Medicine, St. Louis, MO 63110, USA.ORCID 0000-0001-5319-0811
Washington University in St. Louis · US

Funding

MOLECULAR GENETICS OF LEISHMANIA USING DNA TRANSFECTIONR01AI029646 · NIAID · WASHINGTON UNIVERSITY · PI BEVERLEY, STEPHEN M · 1990 to 2015
$9.3M
Leishmania RNA viruses and pathogenesisR01AI130222 · NIAID · WASHINGTON UNIVERSITY · PI BEVERLEY, STEPHEN M · 2018 to 2022
$3.3M
NIAID NIH HHS R01 AI029646NIAID NIH HHS R01 AI130222
6 · The paper itself

Abstract

RNA interference (RNAi) is a powerful tool whose efficacy against a broad range of targets enables functional genetic tests individually or systematically. However, the RNAi pathway has been lost in evolution by a variety of eukaryotes including most Leishmania sp. RNAi was retained in species of the

Indexed as

LeishmaniaLeishmania braziliensisPhenotypeRNA Interferencegene knockdownshypomorphic mutationsintraflagellar transportLeishmania braziliensisLeishmania guyanensisquinonoid dihydropteridine reductase (QDPR)site specific recombinasetrypanosomatid protozoan parasitevirulence

Identifiers

PMID36672832
PMCPMC9858808
OpenAlexW4313238106

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.