ArticleCells2023
Triphasic 3D In Vitro Model of Bone-Tendon-Muscle Interfaces to Study Their Regeneration.
Article in Cells, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed.
- Generation of Human Bioartificial Tissues Using Agarose-Derived Biomaterials.Materials (Basel, Switzerland) · 2026Review
- BiomimeticBioactive materials · 2026Article
- Multicellular microtissues from fused ligament- and bone-cell spheroids relevant to enthesis repair.Materials today. Bio · 2026Article
- Three-dimensional cell sheet model improvesBioactive materials · 2025Article
- 3D Hybrid Bioprinting for Complex Multi-Tissue Engineering.bioRxiv : the preprint server for biology · 2025Article
- Converging Architectures: Precision Biomanufacturing and Soft Robotics Rewiring Tissue Engineering.Micromachines · 2025Article
- LINC01271 promotes fracture healing via regulating miR-19a-3p/PIK3CA axis.Journal of orthopaedic surgery and research · 2025Article
- Hierarchy Reproduction: Multiphasic Strategies for Tendon/Ligament-Bone Junction Repair.Biomaterials research · 2025Review
- Tendon Tissue Engineering: Pathophysiological Mechanism and Bioengineering Therapy of Tendinopathy.International journal of nanomedicine · 2025Review
- The impact of osteoporosis on arthroscopic rotator cuff repair and postoperative tendon-to-bone healing.Frontiers in surgery · 2025Review
- Gradient scaffolds in bone-soft tissue interface engineering: Structural characteristics, fabrication techniques, and emerging trends.Journal of orthopaedic translation · 2025Review
- Calcium Phosphates: A Key to Next-Generation In Vitro Bone Modeling.Advanced healthcare materials · 2024Review
- Engineering interfacial tissues: The myotendinous junction.APL bioengineering · 2024Review
- Synovium-Derived Mesenchymal Stem Cell-Based Scaffold-Free Fibrocartilage Engineering for Bone-Tendon Interface Healing in an Anterior Cruciate Ligament Reconstruction Model.Tissue engineering and regenerative medicine · 2024Article
- Additive manufacturing of poly (lactic acid)/hydroxyapatite/carbon nanotubes biocomposites for fibroblast cell proliferation.Scientific reports · 2023Article
- Multimaterial and multiscale scaffold for engineering enthesis organ.International journal of bioprinting · 2023Article
Corrections and comments
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The transition areas between different tissues, known as tissue interfaces, have limited ability to regenerate after damage, which can lead to incomplete healing. Previous studies focussed on single interfaces, most commonly bone-tendon and bone-cartilage interfaces. Herein, we develop a 3D in vitro model to study the regeneration of the bone-tendon-muscle interface. The 3D model was prepared from collagen and agarose, with different concentrations of hydroxyapatite to graduate the tissues from bones to muscles, resulting in a stiffness gradient. This graduated structure was fabricated using indirect 3D printing to provide biologically relevant surface topographies. MG-63, human dermal fibroblasts, and Sket.4U cells were found suitable cell models for bones, tendons, and muscles, respectively. The biphasic and triphasic hydrogels composing the 3D model were shown to be suitable for cell growth. Cells were co-cultured on the 3D model for over 21 days before assessing cell proliferation, metabolic activity, viability, cytotoxicity, tissue-specific markers, and matrix deposition to determine interface formations. The studies were conducted in a newly developed growth chamber that allowed cell communication while the cell culture media was compartmentalised. The 3D model promoted cell viability, tissue-specific marker expression, and new matrix deposition over 21 days, thereby showing promise for the development of new interfaces.
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Registered trials
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