ArticleBioengineering (Basel, Switzerland)2022
Effect of Biodentine on Odonto/Osteogenic Differentiation of Human Dental Pulp Stem Cells.
Article in Bioengineering (Basel, Switzerland), 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers, 1 of them a synthesis that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
13 citing papers in PubMed, 1 synthesis or guideline pooled it, 12 citations in OpenAlex.
- Regenerative Potential of Biodentine in Complex Endodontic Conditions: A Systematic Review of Clinical and Radiological Evidence.Medicina (Kaunas, Lithuania) · 2026Pooled it
- Cytotoxicity and Bioactivity of Five Hydraulic Silicate Cements in Human Dental Pulp Cells at Different Setting Stages.International dental journal · 2026Article
- Cellular senescence in the dental pulp and its implications for endodontics: a scoping review.Clinical oral investigations · 2026Article
- Article
- Injectable Piezoelectric Hydrogel for Vital Pulp Therapy.Journal of functional biomaterials · 2025Article
- Modulation of primary human apical papilla stem cells: Influence of Enterococcus faecalis, oxygen levels, and calcium silicate-based cements.European journal of oral sciences · 2025Article
- Biodentine Counteracts the Aging Process of Human Dental Pulp Stem Cells Through Wnt/β-Catenin Pathway.International dental journal · 2025Article
- Effect of biodentine modified with bioactive glass nanoparticles on biocompatibility, osteogenic differentiation, and gene expression of human dental pulp stem cells.Journal of conservative dentistry and endodontics · 2025Article
- Odontogenic/osteogenic differentiation of dental pulp stem cells on a Biodentine-coated polymer nanofibers.Biomedical engineering online · 2025Article
- Senescence of dental pulp stem cells: phenotypes, underlying mechanisms and regulatory molecules.Human cell · 2025Review
- Biocompatibility of Hydraulic Calcium Silicate-Based Cement MTA FlowJournal of functional biomaterials · 2025Article
- Effect of Bio MTA plus & ProRoot MTA pulp capping materials on the regenerative properties of human dental pulp stem cells.Scientific reports · 2025Article
- Specimen Shape and Elution Time Affect the Mineralization and Differentiation Potential of Dental Pulp Stem Cells to Biodentine.Journal of functional biomaterials · 2023Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
Abstract
This study aims to compare the biological characteristics of human dental pulp stem cells (hDPSCs) isolated from different-aged populations and examine the effects of Biodentine on proliferation and odonto/osteogenic differentiation of hDPSCs isolated from the elderly in vitro. hDPSCs were isolated from three different-aged populations: group A (≤18 years old), group B (19−59 years old), and group C (≥60 years old). The adhesion, proliferation, odonto/osteogenesis, and senescence were compared. The optimal concentration of aqueous Biodentine extract was determined by CCK-8 assay, alkaline phosphatase (ALP), and alizarin red staining (ARS). The effect of Biodentine on odonto/osteogenic gene and protein expression of hDPSCs in each group was evaluated by quantitative real-time PCR (QRT-PCR) and Western blot. hDPSCs were successfully isolated from three different-aged populations. Flow cytometry revealed that all isolated hDPSCs were positive for CD73 (>90%), CD90 (>90%), CD146 (<30%), and negative for CD45 (<1%). There existed an age-related decline in proliferation, odonto/osteogenic gene expression, and S-phase fraction (p < 0.05), an increase in senescence genes and p21 and p16 expression, and time needed for cell adhesion. Biodentine promoted hDPSC proliferation and mineralization in each group, particularly at a concentration of 0.2 mg/mL. Biodentine markedly enhanced odonto/osteogenesis-related gene and protein expression in each group (p < 0.05). hDPSCs can be obtained from populations of all ages. Though there is an age-related decline in their biological properties, hDPSCs from the elderly still maintain certain proliferation and multidirectional differentiation abilities. Biodentine can significantly promote the proliferation and odonto/osteogenic differentiation of hDPSCs isolated from the elderly over 60 years old, which could be considered a pulp capping material for vital pulp therapy in the elderly. Nevertheless, the efficacy of Biodentine in clinical application has to be further studied.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.