Evidence map›Paper›PMID 36671482›Full record

ArticleBiomolecules2023

Molecular Profiling of Tissue Samples with Chronic Rejection from Patients with Chronic Lung Allograft Dysfunction: A Pilot Study in Cystic Fibrosis Patients.

Francesca Lunardi, Daniela Isabel Abbrescia, Luca Vedovelli, Federica Pezzuto, Francesco Fortarezza, Giovanni Maria Comacchio, Vincenza Guzzardo, Pia Ferrigno, Monica Loy, Chiara Giraudo and 9 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Biomolecules, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

19 authors.

Francesca LunardiDepartment of Cardiac, Thoracic, Vascular Sciences and Public Health, University of Padova, 35128 Padova, Italy.ORCID 0000-0001-5792-4683
Daniela Isabel AbbresciaSchena Foundation, University Hospital of Bari, 70124 Bari, Italy.
Luca VedovelliDepartment of Cardiac, Thoracic, Vascular Sciences and Public Health, University of Padova, 35128 Padova, Italy.ORCID 0000-0003-4847-2333
Federica PezzutoDepartment of Cardiac, Thoracic, Vascular Sciences and Public Health, University of Padova, 35128 Padova, Italy.ORCID 0000-0002-8023-3108
Francesco FortarezzaDepartment of Cardiac, Thoracic, Vascular Sciences and Public Health, University of Padova, 35128 Padova, Italy.ORCID 0000-0002-8954-5888
Giovanni Maria ComacchioDepartment of Cardiac, Thoracic, Vascular Sciences and Public Health, University of Padova, 35128 Padova, Italy.
Vincenza GuzzardoDepartment of Medicine, University of Padova, 35128 Padova, Italy.
Pia FerrignoDepartment of Cardiac, Thoracic, Vascular Sciences and Public Health, University of Padova, 35128 Padova, Italy.
Monica LoyDepartment of Cardiac, Thoracic, Vascular Sciences and Public Health, University of Padova, 35128 Padova, Italy.
Chiara GiraudoDepartment of Medicine, University of Padova, 35128 Padova, Italy.ORCID 0000-0003-0924-5883
Anna Sara FraiaDepartment of Medicine, University of Padova, 35128 Padova, Italy.
Eleonora FaccioliDepartment of Cardiac, Thoracic, Vascular Sciences and Public Health, University of Padova, 35128 Padova, Italy.
Fausto BraccioniDepartment of Cardiac, Thoracic, Vascular Sciences and Public Health, University of Padova, 35128 Padova, Italy.ORCID 0000-0002-7197-9821
Emanuele CozziDepartment of Cardiac, Thoracic, Vascular Sciences and Public Health, University of Padova, 35128 Padova, Italy.
Dario GregoriDepartment of Cardiac, Thoracic, Vascular Sciences and Public Health, University of Padova, 35128 Padova, Italy.ORCID 0000-0001-7906-0580
Geert M VerledenDepartment of Respiratory Diseases, University Hospitals Leuven, 3000 Leuven, Belgium.
Fiorella CalabreseDepartment of Cardiac, Thoracic, Vascular Sciences and Public Health, University of Padova, 35128 Padova, Italy.
Francesco Paolo SchenaSchena Foundation, University Hospital of Bari, 70124 Bari, Italy.ORCID 0000-0001-7927-5207
Federico ReaDepartment of Cardiac, Thoracic, Vascular Sciences and Public Health, University of Padova, 35128 Padova, Italy.

Funding

Italian Cystic Fibrosis Research Foundation 28.2018
6 · The paper itself

Abstract

Chronic rejection (CR) is the main culprit for reduced survival and quality of life in patients undergoing lung transplantation (Ltx). High-throughput approaches have been used to unveil the molecular pathways of CR, mainly in the blood and/or in bronchoalveolar lavage. We hypothesized that a distinct molecular signature characterizes the biopsies of recipients with clinically confirmed histological signs of CR. Eighteen cystic fibrosis patients were included in the study and RNA sequencing was performed in 35 scheduled transbronchial biopsies (TBBs): 5 with acute cellular rejection, 9 with CR, and 13 without any sign of post-LTx complication at the time of biopsy; 8 donor lung samples were used as controls. Three networks with 33, 26, and 36 differentially expressed genes (DEGs) were found in TBBs with CR. Among these, seven genes were common to the identified pathways and possibly linked to CR and five of them (LCN2, CCL11, CX3CL1, CXCL12, MUC4) were confirmed by real-time PCR. Immunohistochemistry was significant for LCN2 and MUC4. This study identified a typical gene expression pattern in TBBs with histological signs of CR and the LCN2 gene appeared to play a central role. Thus, it could be crucial in CR pathophysiology.

Indexed as

Cystic FibrosisAllograftsGraft RejectionHumansLungPilot ProjectsQuality of Lifechronic lung allograft dysfunctionlung transplantationRNA sequencingtransbronchial biopsytranscriptomics

Identifiers

PMID36671482
PMCPMC9856133

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.