Evidence map›Paper›PMID 36671476›Full record

ArticleBiomolecules2023

Construction of Bi-Enzyme Self-Assembly Clusters Based on SpyCatcher/SpyTag for the Efficient Biosynthesis of (R)-Ethyl 2-hydroxy-4-phenylbutyrate.

Jinmei Wang, Yuan Lu, Pengpeng Cheng, Chuyue Zhang, Lan Tang, Lihua Du, Jinghua Li, Zhimin Ou

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Biomolecules, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
2.2field-weighted citation impact, top 13% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 14 citations in OpenAlex.

  1. Article
  2. Review
  3. Article
  4. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 1 institution in 1 country.

Jinmei WangCollege of Pharmaceutical Science, Zhejiang University of Technology, Hangzhou 310014, China.
Yuan LuCollege of Pharmaceutical Science, Zhejiang University of Technology, Hangzhou 310014, China.
Pengpeng ChengCollege of Pharmaceutical Science, Zhejiang University of Technology, Hangzhou 310014, China.
Chuyue ZhangCollege of Pharmaceutical Science, Zhejiang University of Technology, Hangzhou 310014, China.
Lan TangCollege of Pharmaceutical Science, Zhejiang University of Technology, Hangzhou 310014, China.
Lihua DuCollege of Pharmaceutical Science, Zhejiang University of Technology, Hangzhou 310014, China.
Jinghua LiCollege of Pharmaceutical Science, Zhejiang University of Technology, Hangzhou 310014, China.
Zhimin OuCollege of Pharmaceutical Science, Zhejiang University of Technology, Hangzhou 310014, China.
Zhejiang University of Technology · CN

Funding

the National Nature Science Foundation of China 21978267the National Nature Science Foundation of China 22078300the Natural Science Foundation of Zhejiang Province LY15B060005the Natural Science Foundation of Zhejiang Province LY15B060007
6 · The paper itself

Abstract

Cascade reactions catalyzed by multi-enzyme systems are important in science and industry and can be used to synthesize drugs and nutrients. In this study, two types of macromolecules of bi-enzyme self-assembly clusters (BESCs) consisting of carbonyl reductase (CpCR) and glucose dehydrogenase (GDH) were examined. Stereoselective CpCR and GDH were successfully fused with SpyCatcher and SpyTag, respectively, to obtain four enzyme modules, namely: SpyCatcher-CpCR, SpyCatcher-GDH, SpyTag-CpCR, and SpyTag-GDH, which were covalently coupled in vitro to form two types of hydrogel-like BESCs: CpCR-SpyCatcher-SpyTag-GDH and GDH-SpyCatcher-SpyTag-CpCR. CpCR-SpyCatcher-SpyTag-GDH showed a better activity and efficiently converted ethyl 2-oxo-4-phenylbutyrate (OPBE) to ethyl(R)2-hydroxy-4-phenylbutanoate (

Indexed as

TemperatureCyclizationPhenylbutyrates2-hydroxy-4-phenylbutyric acidethyl (R)-2-hydroxy-4-phenylbutyratePhenylbutyratesbi-enzyme self-assembly clusters (BESCs)carbonyl reductase CpCRmulti-enzyme cascade reactions(R)-HPBESpyCatcher/SpyTag

Identifiers

PMID36671476
PMCPMC9855569
OpenAlexW4313472167

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.