Evidence map›Paper›PMID 36670778›Full record

ArticleAnimals : an open access journal from MDPI2023

A New AS-PCR Method to Detect

Gianfranco Cosenza, Sara Albarella, Emanuele D'Anza, Alessandra Iannuzzi, Maria Selvaggi, Mariagiulia Pugliano, Tiziana Galli, Giorgio Saralli, Francesca Ciotola, Vincenzo Peretti

Abstract read
In one paragraph

Article in Animals : an open access journal from MDPI, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed.

  1. TheAnimals : an open access journal from MDPI · 2026
    Article
  2. Association study of theJournal of advanced veterinary and animal research · 2025
    Article
  3. Genes · 2025
    Review
  4. A Comprehensive Analysis ofAnimals : an open access journal from MDPI · 2024
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Gianfranco CosenzaDepartment of Agriculture, University of Napoli Federico II, 80055 Portici, Italy.ORCID 0000-0001-6006-4987
Sara AlbarellaDepartment of Veterinary Medicine and Animal Production, University of Naples Federico II, Via Delpino 1, 80137 Naples, Italy.ORCID 0000-0002-4018-8007
Emanuele D'AnzaDepartment of Veterinary Medicine and Animal Production, University of Naples Federico II, Via Delpino 1, 80137 Naples, Italy.
Alessandra IannuzziNational Research Council (CNR), Institute of Animal Production System in Mediterranean Environment (ISPAAM), Piazzale E. Fermi, 1, 8055 Portici, Italy.ORCID 0000-0002-2693-3111
Maria SelvaggiDepartment of Soil, Plant and Food Sciences, University of Bari Aldo Moro, 70126 Bari, Italy.ORCID 0000-0002-0817-9557
Mariagiulia PuglianoDepartment of Veterinary Medicine and Animal Production, University of Naples Federico II, Via Delpino 1, 80137 Naples, Italy.ORCID 0000-0002-3539-587X
Tiziana GalliIstituto Zooprofilattico Sperimentale Lazio e Toscana "M. Aleandri" (IZSLT), UOT Lazio Sud, Str. Congiunte Destre, 04100 Latina, Italy.
Giorgio SaralliIstituto Zooprofilattico Sperimentale Lazio e Toscana "M. Aleandri" (IZSLT), UOT Lazio Sud, Str. Congiunte Destre, 04100 Latina, Italy.
Francesca CiotolaDepartment of Veterinary Medicine and Animal Production, University of Naples Federico II, Via Delpino 1, 80137 Naples, Italy.
Vincenzo PerettiDepartment of Veterinary Medicine and Animal Production, University of Naples Federico II, Via Delpino 1, 80137 Naples, Italy.

Funding

the Italian Ministry of Health RC IZS LT 10.19
6 · The paper itself

Abstract

Calcium-sensitive caseins are the main protein component of milk. In the goat, they are encoded by three genes (CSN1S1, CSN2, and CSN1S2) located on chromosome 6. A high number of alleles has been discovered for these genes in the goat species, responsible for changes in the milk’s qualitative and quantitative characteristics. This study aimed to develop an Allele-Specific PCR (AS-PCR), which allowed us to unequivocally detect goat carriers of the CSN201 allele. Subsequently, the calcium-sensitive casein loci genotype was investigated in three native goat breeds of the Lazio Region (Bianca Monticellana, Capestrina, and Ciociara Grigia). No individuals were carriers of the CSN1S101, CSN1S1E, CSN201, CSN1S2D, and CSN1S20 alleles, while a high frequency of the alleles CSN1S1F and CSN1S1A*,B* was observed. Association analyses between the different genotypes at the CSN1S1 locus and some milk traits, namely the fat and protein yielded and the fat, protein, solids-not-fat, and casein percentages without an effect on the milk yield, were observed.

Indexed as

autochthonous goat breedscalcium-sensitive caseinsgenotypingmilk traits

Identifiers

PMID36670778
PMCPMC9854881

What OpenQuestion holds

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LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.