Evidence map›Paper›PMID 36662454›Full record

ArticleMolecular biology reports2023

Cytochrome-c-oxidase and ATPsynthase content increases in the endometrium of the patients with adenomyosis.

Konstantin A Toniyan, Elena Yu Gorbacheva, Maria A Golubkova, Victoria V Povorova, Valery V Boyarintsev, Irina V Ogneva

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Article in Molecular biology reports, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.5field-weighted citation impact, top 30% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 2 citations in OpenAlex.

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4 · The record

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PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 1 institution in 1 country.

Konstantin A ToniyanGynecology Department, FGBU KB1 (Volynskaya) UDP RF, 10, Starovolynskaya str, 121352, Moscow, Russia.
Elena Yu GorbachevaGynecology Department, FGBU KB1 (Volynskaya) UDP RF, 10, Starovolynskaya str, 121352, Moscow, Russia.
Maria A GolubkovaCell Biophysics Laboratory, State Scientific Center of the Russian Federation Institute of Biomedical Problems of the Russian Academy of Sciences, 76a, Khoroshevskoyoe shosse, 123007, Moscow, Russia.
Victoria V PovorovaGynecology Department, FGBU KB1 (Volynskaya) UDP RF, 10, Starovolynskaya str, 121352, Moscow, Russia.
Valery V BoyarintsevEmergency and Extreme Medicine Department, FGBU DPO CGMA UDP RF, 19, Marshala Timoshenko str, 121359, Moscow, Russia.
Irina V OgnevaCell Biophysics Laboratory, State Scientific Center of the Russian Federation Institute of Biomedical Problems of the Russian Academy of Sciences, 76a, Khoroshevskoyoe shosse, 123007, Moscow, Russia. iogneva@yandex.ru.ORCID http://orcid.org/0000-0003-0125-5876
Institute of Biomedical Problems · RU

Funding

program for fundamental research of the State Scientific Center of the Russian Federation Institute of Biomedical Problems of the Russian Academy of Sciences 65.4
6 · The paper itself

Abstract

backgroundAdenomyosis is characterized by overgrowth of endometrial glands and stroma in the myometrium and is associated with reduced apoptosis. One of the key participants in one of the pathways of apoptosis is cytochrome c, whose expression can be regulated by actin-binding proteins involved in the formation of structures that provide cell motility. The aim of the study was to determine the content of actin-binding proteins, cytochrome c, and terminal members of the mitochondrial respiratory chain in endometrial biopsies of patients with adenomyosis and the control group. METHODS AND

resultsThe content of all studied proteins was determined by Western blotting, and the mRNA content of the corresponding genes was determined by quantitative RT-PCR. The relative content of alpha-actinin1 and mRNA of the gene encoding it in biopsy specimens from patients with adenomyosis was higher than in controls by 86 and 84% (p < 0.05), respectively. The relative content of alpha-actinin4 did not change, as did cytochrome c. The content of cytochrome-c-oxidase and ATPsynthase in the group with adenomyosis exceeded the control level by 270 and 121% (p < 0.05), respectively, but the relative content of mRNA of these genes did not change, which may indicate a change in regulation at the level of protein synthesis.

conclusionThe results may indicate a local increase in the synthesis of ATP in pathological endometrial cells, which indicates the possible effectiveness of local application of H+-ATP synthase inhibitors (for example, macrolide antibiotic) to reduce the severity of clinical symptoms of adenomyosis.

Indexed as

AdenomyosisEndometriosisAdenosine TriphosphateCytochromes cElectron Transport Complex IVEndometriumFemaleHumansMicrofilament ProteinsRNA, MessengerAdenosine TriphosphateCytochromes cElectron Transport Complex IVMicrofilament ProteinsRNA, MessengerAdenomyosisCytochrome cCytoskeletonEndometriosisReproductive function

Identifiers

PMID36662454
OpenAlexW4317545825

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.