Evidence map›Paper›PMID 36661398›Full record

ArticleJournal of veterinary internal medicine2023

Cancer detection in clinical practice and using blood-based liquid biopsy: A retrospective audit of over 350 dogs.

Andi Flory, Lisa McLennan, Betsy Peet, Marissa Kroll, Deirdre Stuart, Devon Brown, Kathy Stuebner, Brenda Phillips, Brenda L Coomber, J Paul Woods and 18 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of veterinary internal medicine, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.

0numbers the graph read from it
0cells of the map it votes in
8citing papers in PubMed
2.8field-weighted citation impact, top 9% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

8 citing papers in PubMed, 11 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

28 authors at 7 institutions in 3 countries.

Andi FloryPetDx, Inc, La Jolla, California, USA.
Lisa McLennanPetDx, Inc, La Jolla, California, USA.
Betsy PeetPetDx, Inc, La Jolla, California, USA.
Marissa KrollPetDx, Inc, La Jolla, California, USA.
Deirdre StuartDepartment of Clinical Studies, Ontario Veterinary College, University of Guelph, Guelph, Ontario, Canada.
Devon BrownBridge Animal Referral Center, Edmonds, Washington, USA.
Kathy StuebnerClinical Investigation Center, University of Minnesota, Saint Paul, Minnesota, USA.
Brenda PhillipsVeterinary Specialty Hospital of San Diego, San Diego, California, USA.
Brenda L CoomberDepartment of Biomedical Sciences, Ontario Veterinary College, University of Guelph, Guelph, Ontario, Canada.ORCID https://orcid.org/0000-0002-0776-5562
J Paul WoodsInstitute for Comparative Cancer Investigation at the Mona Campbell Centre for Animal Cancer, Ontario Veterinary College, University of Guelph, Guelph, Ontario, Canada.
Mairin MillerVeterinary Specialty Hospital of North County, San Marcos, California, USA.ORCID https://orcid.org/0000-0003-4428-7149
Chelsea D TrippBridge Animal Referral Center, Edmonds, Washington, USA.
Amber Wolf-RingwallDepartment of Veterinary Clinical Sciences, University of Minnesota, College of Veterinary Medicine, Saint Paul, Minnesota, USA.
Kristina M KruglyakPetDx, Inc, La Jolla, California, USA.
Angela L McCleary-WheelerPetDx, Inc, La Jolla, California, USA.
Ashley Phelps-DunnPetDx, Inc, La Jolla, California, USA.
Lilian K WongPetDx, Inc, La Jolla, California, USA.
Chelsea D WarrenPetDx, Inc, La Jolla, California, USA.
Gina BrandstetterPetDx, Inc, La Jolla, California, USA.
Michelle C RosentelPetDx, Inc, La Jolla, California, USA.
Lauren R DiMarzioPetDx, Inc, La Jolla, California, USA.
Allison L O'KellPetDx, Inc, La Jolla, California, USA.
Todd A CohenPetDx, Inc, La Jolla, California, USA.
Daniel S GrosuPetDx, Inc, La Jolla, California, USA.
Jason ChibukPetDx, Inc, La Jolla, California, USA.ORCID https://orcid.org/0000-0003-1903-8829
Dana W Y TsuiPetDx, Inc, La Jolla, California, USA.
Ilya ChornyPetDx, Inc, La Jolla, California, USA.
Jill M RafalkoPetDx, Inc, La Jolla, California, USA.ORCID https://orcid.org/0000-0003-3241-9781
Ambrx (United States) · USEdmonds Community College · USUniversity of Guelph · CAUniversity of Minnesota · USCounty Hospital · GBOntario Institute for Cancer Research · CAOrthopedic Specialty Hospital · US

Funding

UNC-CH CENTER FOR ENVIRONMENTAL HEALTH &SUSCEPTIBILITYP30ES010126 · NIEHS · UNIV OF NORTH CAROLINA CHAPEL HILL · PI Stephanie Engel · 2001 to 2026
$36.3M
6 · The paper itself

Abstract

backgroundGuidelines-driven screening protocols for early cancer detection in dogs are lacking, and cancer often is detected at advanced stages. HYPOTHESIS/

objectivesTo examine how cancer typically is detected in dogs and whether the addition of a next-generation sequencing-based "liquid biopsy" test to a wellness visit has the potential to enhance cancer detection. ANIMALS: Client-owned dogs with definitive cancer diagnoses enrolled in a clinical validation study for a novel blood-based multicancer early detection test.

methodsRetrospective medical record review was performed to establish the history and presenting complaint that ultimately led to a definitive cancer diagnosis. Blood samples were subjected to DNA extraction, library preparation, and next-generation sequencing. Sequencing data were analyzed using an internally developed bioinformatics pipeline to detect genomic alterations associated with the presence of cancer.

resultsIn an unselected cohort of 359 cancer-diagnosed dogs, 4% of cases were detected during a wellness visit, 8% were detected incidentally, and 88% were detected after the owner reported clinical signs suggestive of cancer. Liquid biopsy detected disease in 54.7% (95% confidence interval [CI], 49.5%-59.8%) of patients, including 32% of dogs with early-stage cancer, 48% of preclinical dogs, and 84% of dogs with advanced-stage disease. CONCLUSIONS/CLINICAL IMPORTANCE: Most cases of cancer were diagnosed after the onset of clinical signs; only 4% of dogs had cancer detected using the current standard of care (i.e., wellness visit). Liquid biopsy has the potential to increase detection of cancer when added to a dog's wellness visit.

Indexed as

Dog DiseasesNeoplasmsAnimalsDogsLiquid BiopsyRetrospective Studiescancer screeningclinical signsearly stageincidentalliquid biopsymulticancer early detectionpreclinical

Identifiers

PMID36661398
PMCPMC9889684
OpenAlexW4317567430

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.